Effects of isotretinoin on new bone formation after maxillary sutural expansion
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ORIGINAL ARTICLE
Effects of isotretinoin on new bone formation after maxillary sutural expansion Musa Bulut1
· Yasemin Nur Korkmaz1 · Sevilay Erimsah2
Received: 22 July 2019 / Accepted: 26 November 2019 © Springer Medizin Verlag GmbH, ein Teil von Springer Nature 2019
Abstract Purpose The aim of this study was to examine the effects of isotretinoin on new bone formation after maxillary sutural expansion in rats. Materials and methods A total of 32 male Wistar rats were selected and randomly divided into four groups. The isotretinoin group was treated with 7.5 mg/kg isotretinoin, and the soybean group was treated with 2 ml/kg soybean oil for 57 days. The substances were applied via oral gavage. The expansion-only and the control groups were not treated with any substance. In the experiment groups, expansion springs were applied on day 41 of the experiment, and after day 5 of expansion, a 12-day retention period was established. At the end of the experiment, all the animals were sacrificed, and their maxillae were dissected for histological evaluation. The numbers of osteoclasts, osteoblasts and formation of new bone and capillaries were evaluated on slides centered around the suture. Results The statistical analysis showed significant differences between the groups for the number of osteoblasts and osteoclasts (p < 0.001). In the experiment groups, higher numbers of osteoblasts and osteoclasts were detected in comparison to the control group (p < 0.001), while there was no significant difference between the experiment groups. Capillary formation and new bone formation in the isotretinoin group were found to be on a higher level than in the other groups (p < 0.001). Conclusion Isotretinoin had no negative effects on bone formation following the expansion of the maxillary suture in rats.
Keywords Animal experimentation · Rats · Osteogenesis · Bone development · Palatal expansion technique
Auswirkungen von Isotretinoin auf die Knochenneubildung nach maxillärer suturaler Expansion Zusammenfassung Zielsetzung Ziel der Studie war es, die Auswirkungen von Isotretinoin auf die Knochenneubildung nach maxillärer suturaler Expansion bei Ratten zu untersuchen.
Musa Bulut, DDS, PhD
[email protected] 1
Faculty of Dentistry, Department of Orthodontics, Bolu Abant Izzet Baysal University, Gölköy Yerle¸skesi, 14030 Bolu, Turkey
2
Faculty of Medicine, Department of Histology and Embryology, Bolu Abant Izzet Baysal University, Bolu, Turkey
K
M. Bulut et al.
Materialien und Methoden Insgesamt 32 männliche Wistar-Ratten wurden ausgewählt und zufällig einer von 4 Gruppen zugeordnet. In der Isotretinoin-Gruppe wurde mit 7,5 mg/kg Isotretinoin behandelt, in der Soja-Gruppe mit 2 ml/kg Sojaöl, jeweils über 57 Tage. Die Substanzen wurden über eine orale Sonde appliziert. Die Tiere in der Nur-Expansions- und der Kontrollgruppe erhielten keine Substanzen. In den Versuchsgruppen wurden an Tag 41 Expansionsfedern eingesetzt, nach Tag 5 der Expansion erfolgte eine 12-tägige Retentionsphase. Am Ende des Experiments wurden alle Ti
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