Epitypification of Cercospora coffeicola and its involvement with two different symptoms on coffee leaves in Brazil
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Epitypification of Cercospora coffeicola and its involvement with two different symptoms on coffee leaves in Brazil Paula Adrielly Souza Vale & Mário Lúcio Vilela de Resende & Deila Magna dos Santos Botelho & Camila Cristina Lage de Andrade & Eduardo Alves & Cláudio Ogoshi & Sarah da Silva Costa Guimarães & Ludwig H. Pfenning
Accepted: 17 November 2020 # Koninklijke Nederlandse Planteziektenkundige Vereniging 2020
Abstract Cercospora coffeicola is the causal agent of brown eye spot, an important disease of coffee (Coffea arabica) in Brazil. However, atypical symptom as darker and larger lesions, named black spot, has been reported in field. In this study, we tested the hypothesis that the causal agent of black spot belongs to the same species pathogenic to brown eye spot. Nineteen strains obtained from diseased coffee found in the five largest coffee-producing states of Brazil were characterized by a combination of molecular phylogenic methods, using a multi-locus approach (internal transcribed spacer regions and intervening 5.8S nrRNAs, actin, calmodulin, histone H3, and translation elongation factor 1-alpha),
P. A. S. Vale Faculty of Presidente Antônio Carlos of Teófilo Otoni, Teófilo Otoni, MG 39803-087, Brazil e-mail: [email protected]
analyses of morphological markers and pathogenicity. Strains from brown eye spot and black spot disease on coffee leaves formed a clade with C. coffeicola strain from Japan. All strains showed same morphological characteristics and caused brown eye spot symptoms in greenhouse. These results confirm that the species associated with brown eye spot and black spot disease on coffee leaves is C. coffeicola. Additionally, an epitype is proposed for C. coffeicola.
Keywords Black spot . Brown eye spot . Coffea arabica . Epitype . Multilocus phylogeny . Plant disease
E. Alves e-mail: [email protected]
M. L. V. de Resende : D. M. dos Santos Botelho : E. Alves : S. da Silva Costa Guimarães (*) : L. H. Pfenning Department of Plant Pathology, Federal University of Lavras, UFLA, Lavras, MG 37200-000, Brazil e-mail: [email protected]
L. H. Pfenning e-mail: [email protected]
M. L. V. de Resende e-mail: [email protected]
C. Ogoshi Santa Catarina State Agricultural Research and Rural Extension Agency, Epagri, Caçador, SC 88034-901, Brazil e-mail: [email protected]
D. M. dos Santos Botelho e-mail: [email protected]
C. C. L. de Andrade Agronomica, Av Ipiranga, 7464, conj.1202, Porto Alegre, RS 90230-100, Brazil e-mail: [email protected]
Eur J Plant Pathol
Introduction Coffee (Coffea arabica) is widely cultivated in Brazil, and it is one of the most important agricultural commodities to the Brazilian economy (Souza et al. 2011). Cercospora coffeicola is the causal agent of brown eye spot (BES) disease, which occurs from nursery-to-field plantings, and severe epidemics are associated with coffee yield losses when management is not wellconducted (Souza et al. 2012). It causes lesions on leaves as necrotic spots consisting of a light-colored center
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