Evaluation of biomarkers of joint damage in patients subjected to arthroscopy
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ORIGINAL PAPER
Evaluation of biomarkers of joint damage in patients subjected to arthroscopy Aldo Izaguirre 1 & Guadalupe González-Gutiérrez 2 & Sixto Elmer Galindo-López 3 & Gustavo Arenas-Serna 4 & Amado Rodríguez 5 & Enrique Flores-Torrero 6 & Rebecca Elizabeth Franco y Bourland 7 & Martha Alicia Ballinas-Verdugo 8 & Fausto Sánchez-Muñoz 8 Received: 18 April 2020 / Accepted: 22 September 2020 # SICOT aisbl 2020
Abstract Aims Recently, the determination of biochemical markers has been intensely explored to better understand the mechanisms underlying knee OA. In this study, we aimed to explore the expression pattern of five biochemical markers in patients with knee OA. Methods After IRB approval and signed informed consent, 26 patients were enrolled. Serum and synovial samples were collected prior to knee arthroscopy. Pre-operative assessment included diagnosis, Lysholm, Tegner Activity Scale, IKDC score, and radiographic Kellgren and Lawrence classification. ELISA of CTX-I, CTX-II, NTX-I, MMP3, and MMP13 were measured in serum and synovial fluid samples. Results Twenty-six patients were included, with a mean age of 42 ± 15 years old. Mean results and standard deviation of the biomarkers in serum were as follows: CTX-I 5.8 ± 5.5 ng/mL, CTX-II 3.8 ± 1.7 ng/mL, NTX-I 52 ± 71 (nM BCE), MMP3 1.18 ± 0.6 ng/mL, and MMP13 1243.6 ± 1422 pg/mL; synovial fluid results were as follows: CTX-I 0.74 ± 0.5 ng/mL, CTX-II 5.1 ± 2.5 ng/ mL, NTX-I 254 ± 85 (nM BCE), MMP3 0.4 ± 0.4 ng/mL, and MMP13 797 ± 1391 pg/mL. We observed a differential pattern of expression in serum NTX-I in patients with chronic meniscus injuries when compared with ACL injuries or cartilage lesions. Conclusions In conclusion, the clinical criteria of early OA are useful to categorize patients with knee conditions. The biochemical markers explored did not yield a differential pattern that can be associated with this classification. Serum NTX-I could be a useful marker of chronic meniscal lesion in future longitudinal studies, after adjusting for age and sex. Keywords Knee osteoarthritis . Biomarkers . Clinical criteria
Introduction * Aldo Izaguirre [email protected] 1
Universidad Autónoma de Tamaulipas / Hospital Naval de Tampico, Investigación , Circuito Universitario, 89109 Tampico, Mexico
2
Centro de Rehabilitación Integral Teletón, Altamira, Tamaulipas, Mexico
3
Hospital Naval de Tampico, Tampico, Mexico
4
Hospital Médica Universidad, Tampico, Mexico
5
Hospital Regional Cd. Madero PEMEX, Ciudad Madero, Mexico
6
Sports Clinic Tampico, Tampico, Mexico
7
Instituto Nacional de Rehabilitación, Mexico City, Mexico
8
National Institute of Cardiology, Mexico City, Mexico
Knee osteoarthritis (OA) is the most common joint disease and is associated with significant pain, disability, and elevated costs for its diagnosis and treatment [1]. It is a complex, multifactorial disease with many different phenotypes. Unfortunately, despite the availability of diagnostic and classification criteria for established OA, there is increasing interest in definin
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