Evaluation of HPV-related oral cancer using DNA microarray technology according to the 8th edition of the AJCC staging s
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LETTER TO THE EDITOR
Evaluation of HPV‑related oral cancer using DNA microarray technology according to the 8th edition of the AJCC staging system Ik Jae Kwon1 · Jong Ho Lee1 · Soung Min Kim1 Received: 19 September 2020 / Accepted: 24 September 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Keywords Human papillomavirus (HPV) · Oropharyngeal squamous cell carcinoma (OPSCC) · 8th edition of AJCC · Microarray technology · TNM staging system To the Editor, We read with interest the recently published review article by Machczyński et al. titled “A review of the 8th edition of the AJCC staging system for or oropharyngeal cancer according to HPV status” in the European Archives of Oto-Rhino-Laryngology [1]. This review article was well written and provided a great deal of information regarding oropharyngeal squamous cell carcinoma (OPSCC) staging according to human papillomavirus (HPV) status. However, we would like to suggest a few additional items based on our clinical experiences and recently updated studies. The first comment is about the HPV detection methods described in the 8th edition of the AJCC staging system. In the 8th edition, HPV positivity is determined by p16 immunohistochemistry testing of tumor tissue, which is regarded as a standard method to evaluate HPV-driven carcinogenesis in OPSCC. Machczyński et al. stated that p16 testing alone, without additional complementary assays, may not be specific enough to definitively detect the presence of HPVassociated carcinogenesis and therefore a combination of at least two HPV testing methods may be necessary [1]. We This comment refers to the article available online at https://doi. org/10.1007/s00405-020-05979-9. * Soung Min Kim [email protected]; [email protected] Ik Jae Kwon [email protected] Jong Ho Lee [email protected] 1
Department of Oral and Maxillofacial Surgery, Dental Research Institute, School of Dentistry, Seoul National University, 101 Daehak‑ro, Jongno‑gu, Seoul 110‑768, Korea
strongly agree with this statement and additionally, would like to discuss the importance of HPV subtypes. The classification of HPV types is based mainly on sequence analysis of the L1 gene, which is the most highly conserved gene in all known papilloma viruses [2]. According to this, HPV can be subdivided into low-risk and high-risk types based on oncogenic potential. The high-risk HPV subtypes are HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 54, 56, and 58, and the low-risk subtypes are HPV 6, 11, 34, 40, 42, 43, and 44 [2]. Because detection of various subtypes is important for determining oncogenic aspects and characteristics, we detected HPV DNA by microarray analysis with polymerase chain reaction (PCR). This method can detect HPV at levels well below one copy of the viral genome per cell [3]. The DNA microarray method has sufficient accuracy for detecting known HPV subtypes simultaneously [4]. DNA microarray platforms may be briefly summarized for their capacity to identify the profiles of molecular changes in any particular HPV-related can
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