Expression of wheat Peptide TRansporter 2.1 ( TaPTR2.1 ) during early seed germination
- PDF / 1,434,314 Bytes
- 8 Pages / 595.276 x 790.866 pts Page_size
- 58 Downloads / 173 Views
Plant Biotechnology Reports https://doi.org/10.1007/s11816-020-00639-0
ORIGINAL ARTICLE
Expression of wheat Peptide TRansporter 2.1 (TaPTR2.1) during early seed germination Myoung‑Goo Choi1,2 · Eui Joong Kim1 · Jin Jeon1 · Seok Won Jeong1 · Kyeong‑Hoon Kim2 · Kyeong‑Min Kim2 · Chul Soo Park3 · Chon‑Sik Kang2 · Youn‑Il Park1 Received: 26 July 2020 / Accepted: 19 August 2020 © Korean Society for Plant Biotechnology 2020
Abstract Peptide TRansporters (PTRs) translocate nitrogen (N)-containing substances across cellular membranes. In Arabidopsis thaliana, the seed-abundant, tonoplast-localized AtPTR2 protein is involved in the regulation of water status during seed germination and is negatively regulated by ABA INSENSITIVE4 (ABI4). A survey of publicly available whole-genome sequencing and RNA-seq data revealed that the genomes of hexaploid wheat (Triticum aestivum L.) harbors three homologs of the AtPTR2 gene, TaPTR2.1, TaPTR2.2, and TaPTR2.3, and transcripts of TaPTR2.1 are detected both in dry and germinating seeds, similar to AtPTR2. However, the functional role of TaPTR2.1 during seed germination has not yet been characterized. In the present study, changes in TaPTR2.1 transcript levels were investigated in the wheat cultivar Keumgang in response to phytohormone, sugar, and osmoticum treatments. A transient increase in the transcript level of TaPTR2.1 during seed germination was significantly lowered by abscisic acid (ABA) and PEG4000 treatments. Further, overexpression of the TaPTR2.1 gene under the control of the Cauliflower mosaic virus 35S promoter in the Arabidopsis ptr2 mutant rescued the delayed seed germination phenotype by restoring the seed water status. Together, these results suggest that TaPTR2.1 regulates the water status of wheat seeds during germination in an ABA-dependent manner. Keywords Peptide transporter 2 · Seed germination · Water uptake · Wheat
Introduction
Myong-Goo Choi and Eui Joong Kim contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11816-020-00639-0) contains supplementary material, which is available to authorized users. * Chon‑Sik Kang [email protected] * Youn‑Il Park [email protected] 1
Department of Biological Sciences, Chungnam National University, Daejeon 34134, Republic of Korea
2
National Institute of Crop Science, Rural Development Administration, Wanju 55365, Republic of Korea
3
Department of Crop Science and Biotechnology, Chonbuk National University, Chonju 54896, Republic of Korea
The NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER FAMILY (NPF) of plant proteins is involved in the uptake, transport, and distribution of nitrate, similar to the high-affinity NRT2 nitrate transporters (Tsay et al. 2007; Léran et al. 2014). However, unlike the nitrate-specific NRT2 proteins, NPF proteins also transport other molecules such as peptides, amino acids, phytohormones [auxin and abscisic acid (ABA)], and glucosinolates, in addition to nitrate (see review Corratgé-Faillie and Lacombe 20
Data Loading...
