Seed-specific expression of TaYUC10 significantly increases auxin and protein content in wheat seeds
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ORIGINAL ARTICLE
Seed‑specific expression of TaYUC10 significantly increases auxin and protein content in wheat seeds Yanlin Yang1 · Na Li1 · Wenrong Hui1 · Binjie Yuan1 · Pan Fan1 · Jingxia Liu1 · Honggang Wang1 · Deshun Feng1 Received: 1 September 2020 / Revised: 8 October 2020 / Accepted: 26 October 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Key message Present study revealed that specific expression of TaYUC10.3 in wheat young seeds could increase the content of auxin, and protein. Abstract Auxin is a vital endogenous hormone in plants, which is involved in the regulation of various physiological and biochemical processes in plants. The flavin-containing monooxygenase encoded by the YUCCA gene is a rate-limiting enzyme in the tryptophan-dependent pathway of auxin synthesis. TaYUC10.3 was identified, cloned and found that it was abundantly expressed in wheat young seeds. In this study, a seed-specific expression vector of TaYUC10.3 was constructed with the promoter of 1Bx17 glutenin subunit gene and transformed wheat using the particle bombardment method. The quantitative RT-PCR showed that TaYUC10.3 was expressed in a large amount in young seeds of the transgenic lines. Plant hormone-targeted metabolomics showed that the auxin content of the transgenic lines was significantly increased compared with controls. The GC / MS non-targeted metabolite multiple statistical analyses showed that the variable importance in projection (VIP) of tryptophan reduced in the transgenic lines. Simultaneously, the VIP of indole acetic acid increased. The precursor amino acids for synthesizing some proteins and carbohydrates were upregulated in the transgenic lines. Subsequently, it was found that the protein content of the seeds of the transgenic TaYUC10.3 wheat was significantly higher than that of the control. The wet gluten content and sedimentation value of the transgenic TaYUC10.3 wheat were also high. This result indicated that TaYUC10.3 might participate in auxin synthesis and affects the protein content of wheat seeds. Keywords Wheat · Flavin monooxygenase (YUCCA) · Particle bombardment · Auxin · Metabolome · Protein content Abbreviations ABA Abscisic acid ACC 1-Aminocyclopropanecarboxylic acid EI Electron bombardment ion source ESI Electrospray ion source FC Fold change IAA Indole-3-acetic acid IAA-Ala N-(3-Indolylacetyl)-l-alanine MRM Multiple reaction monitoring
OPLS-DA Orthogonal partial least squares discriminant analysis QC Quality control qRT-PCR Quantitative reverse transcription-polymerase chain reactions RPT Response permutation testing SA Jasmonic acid SA Salicylic acid VIP Variable important in projection ZT Trans-zeatin
Communicated by Chun-Hai Dong. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00299-020-02631-y) contains supplementary material, which is available to authorized users. * Deshun Feng [email protected] 1
State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology
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