First report of Acidovorax avenae subsp. avenae causing bacterial leaf blight of tea in Taiwan
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First report of Acidovorax avenae subsp. avenae causing bacterial leaf blight of tea in Taiwan I-Hsin Sung 1 & Yi-Ru Lai 1 & Chien-Jui Huang 1 Received: 20 March 2020 / Accepted: 28 March 2020 # Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2020
Keywords Camellia sinensis . Acidovorax avenae . Blight . Sequence typing
Severe leaf blight was observed on tea (Camellia sinensis L. cv. Sijichun) with the disease incidence of 60% in commercial orchards in Mingjian, central Taiwan from 2019 to 2020. The infected plants showed brown expanding lesions on the petiole bases of the young leaves and became completely blighted while the disease progressed. A bacterium was consistently isolated on nutrient agar from disinfected symptomatic tissue. Two representative isolates, CsNT01 and CsNT02, were identified by comparison of 16S rDNA sequence and multilocus sequence typing with concatenated sequences of gmc, ugpB, pilT, lepA, trpB, and gltA genes (Feng et al. 2009). The sequences were deposited at GenBank under accession Nos. MT192602–MT192603, and MT199266–MT199277. The 16S rDNA sequences of the isolates shared 99.92% identity to that of A. avenae subsp. avenae type strain ATCC 19860 (EU024134) by BlastN analysis. Phylogenetic analysis of the six concatenated genes revealed the tea isolates well clustered with A. avenae subsp. avenae strains. The tea isolates presented phenotypic characters similar to Acidovorax avenae as determined with API 20 NE system (Biomerieux). For fulfillment of Koch’s postulates, tea cv. Sijichun seedlings were inoculated by immersing leaves in a bacterial suspension (1 × 107 CFU mL−1 in 0.05% Silwet L-77) for 30 s. The inoculated plants were incubated at 28 °C for 3 days in closed plastic bags and subsequently for 4 days on a greenhouse bench. The artificially inoculated tea leaves showed brown lesions indistinguishable from those caused by natural infection. The control leaves remained symptomless. The causal Electronic supplementary material The online version of this article (https://doi.org/10.1007/s42161-020-00547-w) contains supplementary material, which is available to authorized users. * Chien-Jui Huang [email protected] 1
Department of Plant Medicine, National Chiayi University, No. 300, Syuefu Rd, Chiayi City 60004, Taiwan, Republic of China
bacterium was repeatedly isolated from the blighted leaves and confirmed by PCR with the primers Aaaf5, Aaaf3/Aaar2 (Song et al. 2004). Recently, bacterial blight of tea caused by the same pathogen has been reported in China (Geng et al. 2017). To our knowledge, this is the first report of A. avenae subsp. avenae causing bacterial leaf blight of tea in Taiwan.
Acknowledgements This work was financed by the Ministry of Science and Technology (MOST, grant number 108-2313-B-415-006-MY2), Taiwan, R.O.C.
Compliance with ethical standards Conflict of interest The authors declare that they have no conflict of interest. Research involving human participants and/or animals The authors declare that no human participants and
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