Genetically engineered fusion of allergen and viral-like particle induces a more effective allergen-specific immune resp
- PDF / 1,377,068 Bytes
- 15 Pages / 595.276 x 790.866 pts Page_size
- 71 Downloads / 130 Views
BIOTECHNOLOGICAL PRODUCTS AND PROCESS ENGINEERING
Genetically engineered fusion of allergen and viral-like particle induces a more effective allergen-specific immune response than a combination of them Maryam Zamani Sani 1 & Afshar Bargahi 1 & Niloofar Momenzadeh 2 & Parva Dehghani 2 & Maryam Vakili Moghadam 3 & Soheila June Maleki 4 & Iraj Nabipour 2 & Afshin Shirkani 5 & Javad Akhtari 6 & Khashayar Hesamizadeh 7 & Sahel Heidari 3 & Fatemeh Omrani 2 & Samad Akbarzadeh 1 & Mohsen Mohammadi 2 Received: 28 May 2020 / Revised: 31 October 2020 / Accepted: 9 November 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Chimeric virus-like particles (VLPs) were developed as a candidate for allergen-specific immunotherapy. In this study, hepatitis B core antigen (HBcAg) that genetically fused to Chenopodium album polcalcin (Che a 3)–derived peptide was expressed in E. coli BL21, purified, and VLP formation was evaluated using native agarose gel electrophoresis (NAGE) and transmission electron microscopy (TEM). Chimeric HBc VLPs were characterized in terms of their reactivity to IgE, the induction of blocking IgG and allergen-specific IgE, basophil-activating capacity, and Th1-type immune responses. Results from IgE reactivity and basophil activation test showed that chimeric HBc VLPs lack IgE-binding capacity and basophil degranulation activity. Although chimeric HBc VLPs induced the highest level of efficient polcalcin-specific IgG antibody in comparison to those induced by recombinant Che a 3 (rChe a 3) mixed either with HBc VLPs or alum, they triggered the lowest level of polcalcin-specific IgE in mice following immunization. Furthermore, in comparison to the other antigens, chimeric HBc VLPs produced a polcalcinspecific Th1 cell response. Taken together, genetically fusion of allergen derivatives to HBc VLPs, in comparison to a mix of them, may be a more effective way to induce appropriate immune responses in allergen-specific immunotherapy. Key points • The insertion of allergen-derived peptide into major insertion region (MIR) of hepatitis B virus core (HBc) antigen resulted in nanoparticles displaying allergen-derived peptide upon its expression in prokaryotic host. • The resultant VLPs (chimeric HBc VLPs) did not exhibit IgE reactivity with allergic patients’ sera and were not able to degranulate basophils. * Mohsen Mohammadi [email protected]; [email protected] Maryam Zamani Sani [email protected] Afshar Bargahi [email protected] Niloofar Momenzadeh [email protected] Parva Dehghani [email protected] Maryam Vakili Moghadam [email protected] Soheila June Maleki [email protected] Iraj Nabipour [email protected]
Afshin Shirkani [email protected] Javad Akhtari [email protected] Khashayar Hesamizadeh [email protected] Sahel Heidari [email protected] Fatemeh Omrani [email protected] Samad Akbarzadeh [email protected] Extended author information available on the last page of the article
Appl Microbiol Biotechn
Data Loading...
