HIV Protocols
This third edition volume expands upon the previous editions with new research topics and numerous leading-edge HIV protocols. This book contains chapters that cover various aspects of HIV-1 replication and are helpful for investigating the mechanisms of
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Vinayaka R. Prasad Ganjam V. Kalpana Editors
HIV Protocols Third Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
HIV Protocols Third Edition
Edited by
Vinayaka R. Prasad Department of Microbiology & Immunology, Albert Einstein College of Medicine, Bronx, NY, USA
Ganjam V. Kalpana Department of Genetics, Albert Einstein College of Medicine, Bronx, NY, USA
Editors Vinayaka R. Prasad Department of Microbiology & Immunology Albert Einstein College of Medicine Bronx, NY, USA
Ganjam V. Kalpana Department of Genetics Albert Einstein College of Medicine Bronx, NY, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3045-6 ISBN 978-1-4939-3046-3 (eBook) DOI 10.1007/978-1-4939-3046-3 Library of Congress Control Number: 2015960182 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Cover illustration: A human macrophage infected with HIV-1 carrying GFP embedded in Gag Courtesy: Dr. Paul Bieniasz, Aaron Diamond AIDS Research Center and Rockefeller University Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface It has been about 5 years since the publication of the HIV Protocols 2nd edition. Thus, when we were approached by John Walker asking us to compile a new edition, we were pleased to know that previous edition had been quite popular both in print and in terms of online downloads. During the intervening years, there has been a considerable advance in the methods employed in HIV research—especially those pertaining to virology. Therefore, we readily agreed, and now here is HIV Protocols 3rd edition! The protocols included in this book were ch
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