Identification of loci controlling mineral element concentration in soybean seeds
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RESEARCH ARTICLE
Open Access
Identification of loci controlling mineral element concentration in soybean seeds Sidiki Malle1, Malcolm Morrison2 and François Belzile1*
Abstract Background: Mineral nutrients play a crucial role in the biochemical and physiological functions of biological systems. The enhancement of seed mineral content via genetic improvement is considered as the most promising and cost-effective approach compared alternative means for meeting the dietary needs. The overall objective of this study was to perform a GWAS of mineral content (Ca, K, P and S) in seeds of a core set of 137 soybean lines that are representative of the diversity of early maturing soybeans cultivated in Canada (maturity groups 000-II). Results: This panel of 137 soybean lines was grown in five environments (in total) and the seed mineral content was measured using a portable x-ray fluorescence (XRF) spectrometer. The association analyses were carried out using three statistical models and a set of 2.2 million SNPs obtained from a combined dataset of genotyping-bysequencing and whole-genome sequencing. Eight QTLs significantly associated with the Ca, K, P and S content were identified by at least two of the three statistical models used (in two environments) contributing each from 17 to 31% of the phenotypic variation. A strong reproducibility of the effect of seven out these eight QTLs was observed in three other environments. In total, three candidate genes were identified involved in transport and assimilation of these mineral elements. Conclusions: There have been very few GWAS studies to identify QTLs associated with the mineral element content of soybean seeds. In addition to being new, the QTLs identified in this study and candidate genes will be useful for the genetic improvement of soybean nutritional quality through marker-assisted selection. Moreover, this study also provides details on the range of phenotypic variation encountered within the Canadian soybean germplasm. Keywords: Soybean, Minerals, XRF, GWAS, QTL
Background Soybean is utilized for a wide array of food, feed, and industrial purposes, making it one of the most versatile grain crops grown. In fact, soybean is an important source of protein, oils and carbohydrates, as well as other beneficial nutrients such as mineral elements which affect end-use traits of both the oil and protein fractions as well as the quality characteristics of seed * Correspondence: [email protected] 1 Département de phytologie, Faculty of Agricultural and Food Sciences and Institute for Integrative and Systems Biology (IBIS), Laval University, Quebec City, Quebec, Canada Full list of author information is available at the end of the article
used to plant succeeding crops [1]. The availability of mineral nutrients to plants is a very dynamic and complex process that is affected by both biotic and abiotic factors and their interactions [2]. In agriculture, it has been reported that deficiencies in essential elements can lead to yield loss, increased disease susce
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