In Silico Design of a Poly-epitope Vaccine for Urinary Tract Infection Based on Conserved Antigens by Modern Vaccinology
- PDF / 1,696,492 Bytes
- 13 Pages / 595.276 x 790.866 pts Page_size
- 33 Downloads / 152 Views
In Silico Design of a Poly‑epitope Vaccine for Urinary Tract Infection Based on Conserved Antigens by Modern Vaccinology Maryam Javadi1 · Mana Oloomi1 · Saeid Bouzari1 Accepted: 3 November 2020 © Springer Nature B.V. 2020
Abstract Immunoinformatics or vaccine informatics focuses on applying computational approaches to advance vaccine research and development (R&D). It is also considered as an emerging field of research capable of improving immunization programs. Urinary tract infections (UTIs) are induced by exogenous organisms, including Klebsiella pneumonia, Proteus mirabilis, and extra-intestinal pathogenic Escherichia coli (ExPEC), which are closely related strains. Bacterial colonization in the urinary tract causes UTI. The purpose of this study was to design the cross-reactive epitope vaccine for stimulating the immune system (CD8 + and CD4 + T cells) against ExPEC, Klebsiella pneumonia, and Proteus mirabilis. In this study, protective vaccine antigens from the genome of ExPEC were used. Immunological predictions based on informatics were performed using available data to design vaccine candidates for inducing potentially cross-protective T-cells against UTI pathogens. Two vaccine candidates, comprising highly conserved and experimentally verified immunogenic epitopes, were designed. Epitope prediction and selection were performed for nine candidate proteins. The particular fusion of proper linkers, carriers, and epitopes was done, and constructs were designed. Physicochemical characteristics assessment of solubility, allergenicity, and antigenicity; reverse translation and codon optimization; second and third structure predictions were utilized for both vaccine candidates. Finally, the designed constructs with particular conserved epitopes with linkers and carriers were structurally approved. These vaccine candidates could be used as a broad immune system inducer, and its cross-protective immunity in UTI would confirm in the future. Keywords Urinary tract infection (UTI) · In silico · Modern vaccinology · ExPEC · Klebsiella pneumonia · Proteus mirabilis
Introduction In silico vaccine design based on candidate, targets accelerated to facilitate the discovery of new vaccines against pathogens. Vaccine informatics or immune-informatics as an emerging field of research focuses on the applications of computational approaches to improve vaccine research and development (R&D). There are several advantages of subunit vaccines, including purposeful provocation of the immunity system (humoral or cellular), cost-effective, secure, and quick procedure (Flower et al. 2010; He et al. 2010). * Mana Oloomi [email protected] * Saeid Bouzari [email protected] 1
Molecular Biology Department, Pasteur Institute of Iran, Pasteur Ave, 13164 Tehran, Iran
Urinary tract infections (UTIs) are caused by some closely related strains such as extra-intestinal E. coli, Proteus mirabilis, and Klebsiella pneumoniae. The emergence of drug resistance species of these strains would interfere with the proper treatment of UTI (P
Data Loading...
