Influence of Prenatal Hypoxia on the Content of Neuron Specific Enolase in the Structures of the Brain and Blood Serum o
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RIMENTAL ARTICLES
Influence of Prenatal Hypoxia on the Content of Neuron Specific Enolase in the Structures of the Brain and Blood Serum of Rats in Early Ontogeny A. Yu. Morozovaa, A. V. Arutjunyana, d, 1, Yu. P. Milyutinaa, P. Yu. Morozovac, L. S. Kozinad, and I. A. Zhuravinb aOtt
b
Institute of Obstetrics, Gynecology, and Reproductology, St. Petersburg, Russia Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russia c St. Petersburg State University, St. Petersburg, Russia dSt. Petersburg Institute of Bioregulation and Gerontology, St. Petersburg, Russia Received November 18, 2019; revised December 22, 2019; accepted December 27, 2019
Abstract—We studied the dynamics of changes in the content of neuron specific enolase (NSE, EC 4.2.1.11) in the hippocampus, cortex, cerebellum and blood serum of rats at 5th, 10th, and 30th days of postnatal development. It was shown that during the first month of life the content of NSE increases several-fold in all the structures studied, both after hypoxia and in the animals not exposed to hypoxia. It was established that prenatal hypoxia on the 14th day of embryonic development leads to significant changes in the NSE content in all the brain structures studied. In the blood serum, the content of this enzyme is higher in comparison with the control group on the 5th, 10th, and 30th postnatal days in experimental animals. Thus, the lack of oxygen affects the content of this enzyme in both the brain structures and in blood serum of rats, which makes it possible to judge the survival of neurons under conditions of prenatal hypoxia, and also suggests that this index may be used as a marker of fetal CNS disturbance after hypoxia during prenatal development. Keywords: prenatal hypoxia, neuron-specific proteins, neuron specific enolase (NSE), hippocampus, cortex, cerebellum, blood serum, early ontogeny DOI: 10.1134/S1819712420030083
INTRODUCTION In the last decade, there has been an increase in the level of neuropsychiatric diseases, the development of which is associated with pathological processes that occur during embryonic development. The highest frequency of adverse effects was observed in children with intrauterine growth retardation syndrome (IUGR). One of the factors leading to the formation of IUGR is development of chronic placental insufficiency, in which the fetus develops under hypoxic conditions. The duration of hypoxia and the stage of pregnancy when hypoxia occurred determine the formation of the newborn’s central nervous system (CNS). Children with IUGR are characterized by impaired motor function, reduced learning ability, hyperactivity, attention deficit, and other cognitive disturbances. The consequences of prenatal hypoxia are studied using experimental models. It has been shown that hypoxia triggers a number of molecular processes, such as membrane depolariza1 Corresponding
author; address: Mendeleevskaya liniya 3, St. Petersburg, 199034 Russia; e-mail: [email protected].
tion, an increase in i
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