Inhibition of apoptosis by ascorbic and dehydroascorbic acids in Xenopus egg extracts
- PDF / 392,109 Bytes
- 7 Pages / 595.276 x 790.866 pts Page_size
- 97 Downloads / 154 Views
ORIGINAL ARTICLE
Inhibition of apoptosis by ascorbic and dehydroascorbic acids in Xenopus egg extracts Tomohiro Saitoh Æ Yuichi Tsuchiya Æ Toshihiko Kinoshita Æ Motohiro Itoh Æ Shigeru Yamashita
Received: 23 June 2008 / Accepted: 16 September 2008 / Published online: 13 December 2008 Ó Japan Society for Reproductive Medicine 2008
Abstract Purpose The viability of mammalian eggs after ovulation is reported to be improved by the presence of ascorbic acid in the culture medium. However, the pro-survival mechanisms of ascorbic acid are poorly understood. The molecular pathways of apoptosis are evolutionarily conserved among animal species, and Xenopus eggs are technically and ethically more suitable for biochemical analyses than mammalian eggs. We used Xenopus egg cytoplasmic extracts to examine the direct intracellular effects of ascorbic acid. Methods Incubation of egg extracts for more than 4 h induces the spontaneous release of cytochrome c from mitochondria. This event triggers the activation of caspases, cleavage of substrate proteins, and execution of apoptosis. Multiple signal transduction pathways including proteolysis and protein phosphorylation are also involved in this process. We examined whether any of these events might be inhibited by the addition of ascorbic acid. Results Ascorbic acid showed no effect against cytochrome c release, but prevented caspase activation and substrate cleavage. Ascorbic acid also blocked the proteolysis of apoptosis inhibitor proteins and the dephosphorylation of p42 MAP kinase. However, dehydroascorbic
T. Saitoh T. Kinoshita M. Itoh Department of Obstetrics and Gynecology, Toho University Medical Center Sakura Hospital, 564-1 Shimoshizu Sakura-shi, Chiba 285-8741, Japan Y. Tsuchiya (&) S. Yamashita Department of Biochemistry, Toho University School of Medicine, 5-21-16 Omori-nishi Ota-ku, Tokyo 143-8540, Japan e-mail: [email protected]
acid (oxidized form of ascorbic acid) and acetate (unrelated acid) were equally effective, indicating that these effects were primarily due to their acidity. In addition, dehydroascorbic acid inhibited caspase activities directly in vitro. Conclusions The anti-apoptotic effect of ascorbic acid in Xenopus egg extracts is mainly due to cytoplasmic acidification rather than its intracellular antioxidant activity. Instead, oxidative conversion of ascorbic acid into dehydroascorbic acid may inhibit apoptosis through the inhibition of caspases. Keywords Ascorbic acid Apoptosis Caspases Dehydroascorbic acid Xenopus egg extracts
Introduction Aged eggs long after ovulation spontaneously degenerate and die mainly by apoptosis. It is poorly understood how the fate of ovulated eggs is switched from survival to death. Many reports suggest that the viability of isolated oocytes, eggs and early embryos from various animals is improved by the addition of antioxidants into culture medium. For example, ascorbic acid (AA) and/or a-tocopherol prevented the maternal aging and apoptosis of mouse oocytes [1–4]. Compromised oocyte function o
Data Loading...