Xenopus Methods and Protocols
This volume explores techniques used to study and experiment with Xenopus in order to model or understand human disease. The chapters in this book cover topics such as implementation of CRISPR/Cas9 and TALEN in Xenopus from the design
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Kris Vleminckx Editor
Xenopus Methods and Protocols
Methods
in
M o l e c u l a r B i o lo g y
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Xenopus Methods and Protocols
Editor
Kris Vleminckx Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Editor Kris Vleminckx Department of Biomedical Molecular Biology Ghent University Ghent, Belgium
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-8783-2 ISBN 978-1-4939-8784-9 (eBook) https://doi.org/10.1007/978-1-4939-8784-9 Library of Congress Control Number: 2018952461 © Springer Science+Business Media, LLC, part of Springer Nature 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Xenopus Protocols: Methods for Functional Genomics and Disease Modeling The last couple of years have seen unbiased and systematic analysis of gene mutations, genomic rearrangements, and RNA expression in clinical practice, for instance in patients afflicted by Mendelian disorders or cancer. While whole exome and whole genome sequencing (WES and WGS) allow detailed and fast identification of mutations and variants in the afflicted patients, it mostly remains a major challenge to identify the specific genetic defects that are causally linked to the disease. In addition, in order to better understand the molecular basis of the disease, functional models can be required. While some aspects of disease can be functionally addressed in cell culture, induced pluripotent stem cells, or organoids, more complex
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