Isolation and Identification of Saline Tolerance Phosphate-Solubilizing Bacteria Derived from Salt-affected Soils and Th
The salt-affected soils of beach from Tianjin China were sampled to screen the saline tolerance phosphate-solubilizing bacteria (SA-T-PSB) using inorganic phosphorous medium. On basis of the phenotypic characterization and 16S rRNA gene sequencing, 4 isol
- PDF / 267,645 Bytes
- 8 Pages / 439.37 x 666.142 pts Page_size
- 4 Downloads / 226 Views
Isolation and Identification of Saline Tolerance Phosphate-Solubilizing Bacteria Derived from Salt-affected Soils and Their Mechanisms of P-solubilizing Yang Han, Chunmei Wang, Xinglin Li, Xuefei Cao, Aijia Cao and Na Zhao Abstract The salt-affected soils of beach from Tianjin China were sampled to screen the saline tolerance phosphate-solubilizing bacteria (SA-T-PSB) using inorganic phosphorous medium. On basis of the phenotypic characterization and 16S rRNA gene sequencing, 4 isolates with the highest PSA, B1114, B1213, B1303, and J101 were identified as Enterobacter ludwigii, Pantoea ananatis, Pseudomonas psychrotolerans, and Gluconobacter frateurii, respectively. Subsequently, aimed to assaying the mechanisms of P-solubilizing, organic acid types of the 4 isolates were determined by high performance liquid chromatography. The results showed that all 4 isolates mainly secreted gluconic acid. The effect of carbon and nitrogen source on P-solubilization activity of the strains also indicated that the production of gluconic acid is the main mechanisms of P-solubilizing.
Keywords Microorganism isolation Microorganism identification solubilizing bacteria P-solubilizing mechanisms
Phosphate-
135.1 Introduction Phosphorus as the most essential macronutrients beside N is the second largest agricultural fertilizer limiting the crop yields [1]. However, many soils in the world are P-deficient and thus cannot sustain the good crop yield [2]. Total P in soil generally ranges from 400 to 1,200 mg/kg which is far beyond the plant’s requirement [3], but only a small proportion (about 0.1 %) can be efficiently utilized by plant [4]. Most P is precipitated with cations (Ca, Fe, Al) in the form of insoluble P which is unavailable to plant [5]. So the chemical P fertilizer is applied Y. Han C. Wang X. Li (&) X. Cao A. Cao N. Zhao Key Lab of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, People’s Republic of China e-mail: [email protected]
T.-C. Zhang et al. (eds.), Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012), Lecture Notes in Electrical Engineering 250, DOI: 10.1007/978-3-642-37922-2_135, Springer-Verlag Berlin Heidelberg 2014
1259
1260
Y. Han et al.
to yield, but a large proportion of available phosphate is rapidly transformed into its poorly soluble forms before plant absorbing it [6]. Microorganisms are considered to play a fundamental role in P cycle by biologically mediated processes. Phosphate solubilizing bacteria (PSBs) could dissolve fixed soil P to soluble form which is utilized by plant. Many reports showed that the application of PSBs could increase available P in soil and improve crop yields [7]. But environmental factors might affect severely on the colonization and performance of PSBs. Especially under stress of hash environment, introduced PSBs cannot survive in quantity or compete with the native organisms in plant rhizosphere [5]. In China, the saline-alkali soil acco
Data Loading...
