Endophytic bacteria associated with halophyte Seidlitzia rosmarinus Ehrenb. ex Boiss. from saline soil of Uzbekistan and
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Springer-Verlag
Endophytic bacteria associated with halophyte Seidlitzia rosmarinus Ehrenb. ex Boiss. from saline soil of Uzbekistan and their plant beneficial traits Vyacheslav SHURIGIN1*, Dilfuza EGAMBERDIEVA2,3, LI Li2, Kakhramon DAVRANOV4, Hovik PANOSYAN5, Nils-Kåre BIRKELAND6, Stephan WIRTH3, Sonoko Dorothea BELLINGRATH-KIMURA3,7 1
Faculty of Biology, National University of Uzbekistan, Tashkent 100174, Uzbekistan; Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China; 3 Leibniz Centre for Agricultural Landscape Research (ZALF), Müncheberg 15374, Germany; 4 Institute of Microbiology of the Academy of Sciences of the Republic of Uzbekistan, Tashkent 100128, Uzbekistan; 5 Department of Biochemistry, Microbiology and Biotechnology, Yerevan State University, Yerevan 0025, Armenia; 6 Department of Biological Sciences, University of Bergen, NO-5020 Bergen 7803, Norway; 7 Faculty of Life Science, Humboldt University of Berlin, Berlin 14195, Germany
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Abstract: Endophytic bacteria of halophytic plants play essential roles in salt stress tolerance. Therefore, an understanding of the true nature of plant–microbe interactions under extreme conditions is essential. The current study aimed to identify cultivable endophytic bacteria associated with the roots and shoots of Seidlitzia rosmarinus Ehrenb. ex Boiss. grown in the salt-affected soil in Uzbekistan and to evaluate their plant beneficial traits related to plant growth stimulation and stress tolerance. Bacteria were isolated from the roots and the shoots of S. rosmarinus using culture-dependent techniques and identified by the 16S rRNA gene. RFLP (Restriction Fragment Length Polymorphism) analysis was conducted to eliminate similar isolates. Results showed that the isolates from the roots of S. rosmarinus belonged to the genera Rothia, Kocuria, Pseudomonas, Staphylococcus, Paenibacillus and Brevibacterium. The bacterial isolates from the shoots of S. rosmarinus belonged to the genera Staphylococcus, Rothia, Stenotrophomonas, Brevibacterium, Halomonas, Planococcus, Planomicrobium and Pseudomonas, which differed from those of the roots. Notably, Staphylococcus, Rothia and Brevibacterium were detected in both roots and shoots, indicating possible migration of some species from roots to shoots. The root-associated bacteria showed higher levels of IAA (indole-3-acetic acid) synthesis compared with those isolated from the shoots, as well as the higher production of ACC (1-aminocyclopropane-1-carboxylate) deaminase. Our findings suggest that halophytic plants are valuable sources for the selection of microbes with a potential to improve plant fitness under saline soils. Keywords: endophytic bacteria; phylogenetic analysis; halophyte; auxin; plant beneficial traits Citation: Vyacheslav SHURIGIN, Dilfuza EGAMBERDIEVA, LI Li, Kakhramon DAVRANOV, Hovik PANOSYAN, Nils-Kåre BIRKELAND, Stephan WIRTH, Sonoko Dorothea BELLINGRATH-KIMURA. 2020. Endophytic bacteria associated with h
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