Kisspeptin-10 inhibits proliferation and regulates lipolysis and lipogenesis processes in 3T3-L1 cells and isolated rat
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ORIGINAL ARTICLE
Kisspeptin-10 inhibits proliferation and regulates lipolysis and lipogenesis processes in 3T3-L1 cells and isolated rat adipocytes Ewa Pruszyńska-Oszmałek1 Paweł A. Kołodziejski1 Maciej Sassek1 Joanna H. Sliwowska2 ●
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Received: 29 July 2016 / Accepted: 20 January 2017 © Springer Science+Business Media New York 2017
Abstract Introduction Kisspeptin, which is encoded by the KISS1 gene and acts via GPR54, plays a role in the regulation of reproductive functions. Expression of KISS1 and GRPR54 has been found in peripheral tissues, including adipose tissue, and was shown to be influenced by metabolic status. Purpose We hypothesized that kisspeptin could be involved in regulation of lipid metabolism in the mouse 3T3-L1 cell line and in isolated rat adipocytes. Methods First, we characterized expression profiles of KISS1 and GPR54 mRNA and proteins in adipose cells isolated from male rats. Secondly, we studied the effects of kisspeptin-10 on cell proliferation and survival in 3T3-L1 cells. Thirdly, we assessed the rapid action of kisspeptin-10 on lipid metabolism and glucose uptake using 3T3-L1 cells and rat primary adipocytes. Finally, we examined the effects of kisspeptin-10 on the secretion of leptin and adiponectin in rat adipocytes. Results We have found that: (1) KISS1 and GPR54 were expressed in mouse 3T3-L1 cells and isolated rat adipocytes; (2) kisspeptin-10: (i) inhibited cell proliferation, viability and adipogenesis in 3T3-L1 and decreased expression of PPAR-γ and CEBPβ—genes, which are involved in the differentiation processes and adipogenesis; (ii) increased lipolysis in 3T3-L1 cells and rat adipocytes by enhancing expression of periliphin and hormone-sensitive
* Joanna H. Sliwowska [email protected] 1
Department of Animal Physiology and Biochemistry, Poznan University of Life Sciences, Wołynska 33, 60-625 Poznan, Poland
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Laboratory of Neurobiology, Institute of Zoology, Poznan University of Life Sciences, Wojska Polskiego 71C, 60-625 Poznan, Poland
lipase; (iii) modulated glucose uptake and lipogenesis; (iv) stimulated leptin and decreased adiponectin secretion from rat adipocytes. Conclusion Kisspeptin-10 could play a role in the regulation of lipid metabolism in mouse 3T3-L1 cells and rat adipocytes. Keywords Kisspeptin GPR54 Adipocytes 3T3-L1 cells ●
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Introduction Kisspeptin, which is encoded by KISS1 gene and acts via receptor GPR54 (KISS1R), has appeared as a major regulator of the reproductive functions [1]. Reproduction is a costly process in terms of energy consumption. Information regarding metabolic status is signaled to the hypothalamus through peripheral peptides from the adipose tissue and gastrointestinal tract [2, 3]. Besides the central expression of KISS1 and its receptor in the hypothalamus, it is also expressed in peripheral tissues including the adipose tissue [4–6]. Kiss1 mRNA and kisspeptin have been detected in rat adipose tissue [7] and KISS1 expression was modulated by nutritional status [4]. Food restriction (18 h) increased Kiss1 mRNA in
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