Meta-DNA Strategy to Assemble DNA Structures in Submicrometre and Micrometre Scale
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doi:10.1007/s40242-020-0352-3
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Meta-DNA Strategy to Assemble DNA Structures in Submicrometre and Micrometre Scale KONG Deming* Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Centre for Analytical Sciences, College of Chemistry, Nankai University, Tianjin 300071, P. R. China Abstract To break through the size limitation in DNA structure assembly and to achieve the construction of DNA structures with submicrometre and micrometre scale, Fan and Yan et al. proposed a versatile DNA structure assembly strategy, named as meta-DNA(M-DNA) strategy. Submicrometre-sized M-DNA, which has a six-helix bundle DNA origami nanostructure, is used as building block to precisely assemble a series of submicrometre-to-micrometre-sized DNA architectures with customed shapes, including meta-multi-arm junctions, polyhedrons and closely packed lattices. Besides static assembly, the proposed M-DNA strategy was also demonstrated to work well for the programmed dynamic rearrangement of DNA structures. This work has been published online in the Nature Chemistry on September 7, 2020. Predictable base-pairing rule and rigid-flexible structural feature confer DNA with powerful self-assembly ability. With the invention and development of DNA origami technology, a milestone in DNA nanotechnology, various customized DNA nanostructures and functional devices have been successfully constructed using DNA as building blocks[1,2]. However, the size of the reported DNA architectures so far is mainly limited to a few nanometers to one or two hundred nanometers. It is still a huge challenge to assemble DNA structures with larger sizes(micrometres to millimetres), which severely limits the widespread use of DNA origami technology. To address this issue, Fan and Yan et al.[3] recently
Fig.1
developed a ‘meta-DNA’(M-DNA) strategy and have achieved the static and dynamic assembly of DNA structures with submicrometre and micrometre scale. In the proposed strategy, M-DNA, a six-helix DNA origami bundle with 6 nm×6 nm in thickness(comparable to that of hair) and ca. 420 nm in length (about 1000 times original DNA nanostructure), is prepared to work as a magnified analogue of ssDNA(Fig.1). The fascinating properties, such as complementary base pairing capability, programmable flexibility and rigidity, and three-dimensional (3D) arrangement of bases allow M-DNA to assemble into large-sized DNA structures in a similar manner to that used in short ssDNA-based DNA nanostructure assembly.
Assembly of nanometer-sized scaffold origami using short ssDNAs and submicrometre-tomicrometre-sized DNA architectures using M-DNA[3]
Using M-DNA as building blocks, a series of submicrometre-to-micrometre-sized DNA architectures, including one-dimensional(1D) DNA lines, two-dimensional(2D) metajunctions and meta-double-crossover tiles, 3D polyhedrons, prisms and closely packed lattices were successfully assembled and demonstrated by agarose gel electrophoresis, atomic force
microscope(AFM) and transmission electron microscope(TEM) characterizat
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