Microbiological contamination in donor corneas preserved for medium-term
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Microbiological contamination in donor corneas preserved for medium-term Gui-gang Li . Hui Zhu . Cai-ni Ji . Xin-jie Zang
Received: 28 September 2018 / Accepted: 13 June 2019 Ó Springer Nature B.V. 2019
Abstract To evaluate the characteristics of microbiological contamination in donor corneas preserved for medium-term. A total of 82 donated corneas from June 1, 2014 to November 30, 2014 were retrospectively analyzed. The corneas were preserved in cornea chambers medium-term solution at 4–8 °C for keratoplasty. After removal of the central corneas for transplantation, the corneoscleral rims were put back into the medium for 1 month at room temperature (20–25 °C). The suspicious contaminated storage solutions indicated with transparency or color change
Gui-gang Li and Hui Zhu have contributed equally to this paper.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10561-019-09776-5) contains supplementary material, which is available to authorized users. G. Li H. Zhu Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China C. Ji Department of Ophthalmology, Third Hospital of Wuhan, Wuhan 430060, Hubei Province, China
were examined with bacteria and fungi cultivation for strain identification. The data collected included gender, age, procurement site and causes of death of donors, and follow-up of recipients. Statistical analysis was performed using Microsoft Excel and SPSS 24.0. Significance level was set at a P value \ 0.05. The overall pathogen positive rate was 9.8% (n = 8), including 7 (87.5%) fungi and 1 (12.5%) bacteria. They were 2 (2.44%) Fusarium, 2 (2.44%) Chromomycosis, 1 (1.22%) Candida albicans, 1 (1.22%) Aspergillus versicolor, 1 (1.22%) Acremonium species, and 1 (1.22%) Enterococcus. 5 contaminated corneas were used for penetrating keratoplasty; although four out of five (80%) had not been given antifungal drugs during more than 6 months following-up period, none of the recipients was infected with a graft. Donor age (P = 0.839), gender (P = 0.062), procurement sites (P = 0.713) and cause of death (P = 0.711) had no statistically significant influence on the contamination rate. All donor corneas have a possibility of microbiological contamination. Strict tissue preservation protocol but not antifungal drugs following keratoplasty seems necessary to prevent graft infection. Keywords Donor cornea Microbiological contamination Eye banking
X. Zang (&) Department of Ophthalmology, Qilu Hospital of Shandong University (Qingdao), Qingdao 266035, Shandong Province, China e-mail: [email protected]
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Introduction Corneal disease is the second major cause of blindness worldwide. In China, there are about 3 million patients suffering from corneal blinding, with 200,000 new blind patients added each year (Shi and Xie 2014). Due to the improvement of the ophthalmology microsurg
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