Molecular cloning and expression analysis of mc5r like genes ( mc5rl ) in Ruditapes philippinarum (Manila clam) after ae
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ORIGINAL ARTICLE
Molecular cloning and expression analysis of mc5r like genes (mc5rl) in Ruditapes philippinarum (Manila clam) after aerial exposure and low‑temperature stress Kunyin Jiang1,2 · Hongtao Nie1,2 · Xiwu Yan1,2 Received: 21 June 2020 / Accepted: 22 October 2020 © Springer Nature B.V. 2020
Abstract The melanocortin-5 receptor (mc5r) plays an important role in exocrine function, lipid metabolism, obesity, and stress response in the vertebrate. However, the functions of the mc5r in mollusks have been rarely investigated. We cloned the full length of Ruditapes philippinarum mc5r like gene (mc5rl) and the sequence structure and phylogenetic relationship of mc5rl were analyzed. Besides, we detected the tissue distribution and the expression pattern of R. philippinarum mc5r like (mc5rl) genes after aerial exposure and low-temperature stress. The full-length cDNA of the mc5rl-1 was 2143 bp, consisting of a 1224 bp open reading frame encoding (ORF) 408 amino acids. Sequence and phylogenetic analyses revealed that the nucleotide and amino acid sequences of Manila clam mc5rl were highly homologous with mc5r of Crassostrea virginica, Crassostrea gigas, Mizuhopecten yessoensis, and Pecten maximus (32%-36%) and low homologous with vertebrates. The results of the distribution of mc5rl genes showed that mc5rl genes were dominant in the mantle, gonad, and hepatopancreas in R. philippinarum. The expression of mc5rl genes was significantly increased after aerial exposure and low-temperature stress in R. philippinarum in hepatopancreas. Aerial exposure and low-temperature stress could induce mc5rl expressed. Mc5rl might serve as a sensor and promote stress response in R. philippinarum. The cloning and expression characteristics of mc5rl will facilitate the investigation of its function in stress response and other physiological processes in R. philippinarum. Keywords Melanocortin-5 receptor like genes (mc5rl) · Cloning · Ruditapes philippinarum · Stress response
Introduction The melanocortin (MC) system consists of melanocyte-stimulating hormone, adrenocorticotropic hormone, and their receptors [1]. Melanocortin receptors (mcrs) are encoded by a gene family consisting of five members (mc1r-mc5r) [2]. Several cellular functions such as skin pigmentation [3], steroidogenesis [4], energy homeostasis [5, 6], and Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11033-020-05941-2) contains supplementary material, which is available to authorized users. * Hongtao Nie [email protected] 1
College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China
Engineering Research Center of Shellfish Culture and Breeding in Liaoning Province, Dalian Ocean University, Dalian 116023, China
2
regulatory immunity [7, 8] regulate by Mcrs have been identified. Mcrs are numbers of rhodopsin family and belong to G protein-coupled receptors (GPCRs), which consist of seven trans-membrane domains (TMs) [9–11]. GPCRs constitute one of the largest and most ancient sup
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