Molecular phylogeny and morphology reveal cryptic species in Blackwellomyces and Cordyceps (Cordycipitaceae) from Thaila
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ORIGINAL ARTICLE
Molecular phylogeny and morphology reveal cryptic species in Blackwellomyces and Cordyceps (Cordycipitaceae) from Thailand Suchada Mongkolsamrit 1 & Wasana Noisripoom 1 & Kanoksri Tasanathai 1 & Artit Khonsanit 1 & Donnaya Thanakitpipattana 1 & Winanda Himaman 2 & Noppol Kobmoo 1 & Janet Jennifer Luangsa-ard 1 Received: 21 May 2020 / Revised: 5 August 2020 / Accepted: 7 August 2020 # German Mycological Society and Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Nine species in Cordyceps sensu lato producing orange to red stromata were discovered during diversity surveys of arthropodpathogenic fungi in Thailand. The phylogenetic analyses of combined ITS, LSU, RPB1, RPB2 and TEF1 sequence data indicated four novel species belonging to Blackwellomyces and five novel species of Cordyceps. All Blackwellomyces species produce filiform ascospores with septations. Blackwellomyces aurantiacus and Blackwellomyces roseostromatus occur on lepidopteran larvae, and both species produce an Evlachovaea-like conidial arrangement of the anamorph and produce a red pigment that diffuses across agar medium. Blackwellomyces calendulinus and Blackwellomyces minutus are found on coleopteran larvae producing Acremonium-like and Mariannaea-like conidial arrangements of their anamorphs and do not produce any pigment. Novel members of Cordyceps include the species Cordyceps brevistroma, Cordyceps inthanonensis, Cordyceps neopruinosa, and Cordyceps parvistroma that are pathogenic on lepidopteran larvae and cocoons, whereas C. araneae is pathogenic on spiders. All Cordyceps species produce bola-shaped, whole ascospores, except for C. inthanonensis that produces ascospores dissociating into part-spores. A common feature of the novel Cordyceps species presented herein is the production of Evlachovaea-like or Mariannaea-like conidial arrangement of the anamorphs. Keywords Cordycipitaceae . Phylogeny . New taxa . Taxonomy
Introduction Most species in the Cordycipitaceae Kreisel ex G.H. Sung, J.M. Sung, Hywel-Jones & Spatafora are pathogenic to a wide range of arthropods. They can be found in the leaf litter, underside of leaves, on stems of plants, or with hosts buried in the ground (Sanjuan et al. 2014; Chen et al. 2018; Mongkolsamrit et al. 2018; Zha et al. 2019; Khonsanit et al. 2020; Thanakitpipattana et al. 2020). Arthropod-pathogenic fungi in Cordycipitaceae are easy to recognize as they have Section Editor: Marc Stadler * Janet Jennifer Luangsa-ard [email protected] 1
National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand
2
Forest Entomology and Microbiology Research Group, Forest and Plant Conservation Research Office, 61 Department of National Parks, Wildlife and Plant Conservation, Phaholyothin Road, Chatuchak, Bangkok 10900, Thailand
pallid to brightly coloured, fleshy stromata. These fertile stromata possess either superficial, semi-immersed, to completely immersed perithecia. The
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