Multiple Myeloma Methods and Protocols

This volume provides a thorough guide of the study of multiple myeloma (MM)—from sample acquisition to the performance of molecular tests, and also discusses difficulties that arise during quality control and interpretation of results. The chapters in thi

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Christoph Heuck Niels Weinhold Editors

Multiple Myeloma Methods and Protocols

Methods

in

M o l e c u l a r B i o lo g y

Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK

For further volumes: http://www.springer.com/series/7651

Multiple Myeloma Methods and Protocols

Edited by

Christoph Heuck Janssen R&D, Spring House, PA, USA

Niels Weinhold The Myeloma Institute, University of Arkansas for Medical Science, Little Rock, AR, USA

Editors Christoph Heuck Janssen R&D Spring House, PA, USA

Niels Weinhold The Myeloma Institute University of Arkansas for Medical Science Little Rock, AR, USA

ISSN 1064-3745     ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7864-9    ISBN 978-1-4939-7865-6 (eBook) https://doi.org/10.1007/978-1-4939-7865-6 Library of Congress Control Number: 2018940666 © Springer Science+Business Media, LLC, part of Springer Nature 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.

Preface This special edition is aimed at molecular scientist and laboratory supervisors working with multiple myeloma (MM) samples and those investigators who would like to embark on studies in this field. MM is a complex disease that in most cases cannot be cured. It not only involves genomic and phenotypic alterations of plasma cells but also implicates the entire bone marrow microenvironment. The complex nature of the pathogenesis of this disease demands that great care is taken during the acquisition, isolation, and archiving of samples and the choice of molecular methods for the analysis of target cell populations, in