N -acyl-homoserine lactones (AHLs) in intertidal marsh: diversity and potential role in nitrogen cycling
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N-acyl-homoserine lactones (AHLs) in intertidal marsh: diversity and potential role in nitrogen cycling Zongxiao Zhang & Yanling Zheng & Ping Han & Hongpo Dong & Xia Liang & Guoyu Yin & Dianming Wu & Yi Yang & Sitong Liu & Min Liu & Lijun Hou
Received: 28 November 2019 / Accepted: 6 July 2020 # Springer Nature Switzerland AG 2020
Abstract Aims N-acyl-L-homoserine lactones (AHLs) based quorum sensing (QS) phenomenon is recognized as an effective agent for regulating bacterial growth and metabolism. However, diversity and biological role of AHLs in natural environments remain largely unknown. This study focuses on compositions of AHLs and their potential role in nitrogen transformation in intertidal marshes. Methods We investigated the levels of AHLs in rhizosphere (Phragmites australis, Spartina alterniflora and Scirpus mariqueter) and non-rhizosphere soils from Chongming eastern intertidal wetland of the Yangtze Estuary using gas chromatography-mass spectrometry (GC/MS). Molecular techniques were employed to investigate the compositions and structure of bacterial Responsible Editor: Paul Bodelier. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11104-020-04630-0) contains supplementary material, which is available to authorized users. Z. Zhang : Y. Zheng : P. Han : Y. Yang : L. Hou (*) State Key Laboratory of Estuarine and Coastal Research, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China e-mail: [email protected] Y. Zheng : P. Han : H. Dong : X. Liang : G. Yin : D. Wu : Y. Yang : M. Liu College of Geographical Sciences, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China S. Liu Department of Environmental Engineering, Peking University, Beijing 100871, China
community. Soil-slurry experiments in combination with nitrogen isotope tracing technique were conducted to examine the effects of AHLs on nitrogen transformation processes. Results AHLs were higher in rhizosphere than nonrhizosphere soils, and correlated significantly with soil organic carbon and pH. C6-, C8-, C10- and C12-HSLs were identified as the probably universal QS signal molecules in intertidal marsh soils. The bacterial biodiversity might be regulated by AHLs, and 12 of the 50 most abundant microorganisms in intertidal marsh soils were related significantly with AHL concentrations. Furthermore, C6-, C8- and C12-HSLs might play an important role in mediating nitrogen transformation processes including nitrification, denitrification and anaerobic ammonium oxidation (anammox). Conclusions AHLs are favored to accumulate in rhizospheric environments and thus further mediate the bacterial diversity and soil nitrogen cycling. This study highlights the associations of QS signal molecules with the dynamics and activity of nitrogen cycling bacteria in intertidal marshes. Keywords AHLs . Quorum sensing . Intertidal marsh . Microbial community . Nitrogen cycle
Introduction Quorum sensing (QS), cell-to-cell communication, refers to a self-regulat
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