Community diversity and distribution of ammonia-oxidizing archaea in marsh wetlands in the black soil zone in North-east
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RESEARCH ARTICLE
Community diversity and distribution of ammonia-oxidizing archaea in marsh wetlands in the black soil zone in North-east China Chunhong Chen1, Hong Liang (✉)2, Dawen Gao (✉)1,2 1 State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China 2 School of Environment, Harbin Institute of Technology, Harbin 150090, China
HIGHLIGHTS
GRAPHIC ABSTRACT
• AOA amoA genes in the soils of the two wetlands affiliated with three lineages. • The main drivers of AOA community were pH and total organic carbon and ammonium. • The soil characteristics rather than the vegetation control the AOA community.
ARTICLE INFO Article history:
Received 22 March 2019 Revised 22 April 2019 Accepted 25 April 2019 Available online 10 July 2019 Keywords: Ammonia-oxidizing archaea amoA gene Freshwater marsh Diversity Distribution
ABSTRACT Since its first detection, ammonia-oxidizing archaea (AOA) have been proven to be ubiquitous in aquatic and terrestrial ecosystems. In this study, two freshwater marsh wetlands- the Honghe wetland and Qixinghe wetland – in the black soil zone in North-east China were chosen to investigate the AOA community diversity and distribution in wetland soils with different vegetation and depth. In the Honghe wetland, two sampling locations were chosen as the dominant plant transited from Deyeuxia to Carex. In the Qixinghe wetland, one sample location that was dominated by Deyeuxia was chosen. Samples of each location were collected from three different depths, and Illumina MiSeq platform was used to generate the AOA amoA gene archive. The results showed that the AOA amoA genes in the soils of the two wetlands were affiliated with three lineages: Nitrososphaera, Nitrosotalea, and Nitrosopumilus clusters. The different dominant status of these AOA lineages indicated their differences in adapting to acidic habitat, oxygenic/hypoxic alternation, organic matter, and other environmental factors, suggesting high diversity among AOA in marsh soils. The main driver of the AOA community was pH, along with organic carbon and ammonium nitrogen, which also played an important role combined with many other environmental factors. Thus, soil physiochemical characteristics, rather than vegetation, were the main cause of AOA community diversity in the wetlands in the black soil zone in China. © Higher Education Press and Springer-Verlag GmbH Germany, part of Springer Nature 2019
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Introduction
Nitrogen cycle is one of the most important material exchange processes that affect the production of ecological
✉ Corresponding authors E-mail: [email protected] (H. Liang); [email protected] (D. Gao)
system on earth. Since most of the nitrogen cycle processes in nature involve microorganisms, microbial ammonia oxidizers that perform the rate-limiting step of nitrogen cycle (ammonia oxidation) have attracted widespread attention (Jetten, 2008; Kuypers et al., 2018; Ward and Jensen, 2014). Identification of ammonia-oxidizing archaea (AOA) had historically changed the unders
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