Non-infected preterm parturition is related to increased concentrations of IL-6, IL-8 and MCP-1 in human cervix
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BioMed Central
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Non-infected preterm parturition is related to increased concentrations of IL-6, IL-8 and MCP-1 in human cervix Susanne Abelin Törnblom1, Aurelija Klimaviciute*1, Birgitta Byström1, Milan Chromek2, Annelie Brauner2 and Gunvor Ekman-Ordeberg1 Address: 1Dept of Women and Child Health, Division for Obstetrics and Gynecology, Karolinska Institute, Karolinska University Hospital Solna, 171 76 Stockholm, Sweden and 2Microbiology and Tumor Biology Center, Division for Clinical Microbiology, Karolinska Institute, Karolinska University Hospital Solna, 171 76 Stockholm, Sweden Email: Susanne Abelin Törnblom - [email protected]; Aurelija Klimaviciute* - [email protected]; Birgitta Byström - [email protected]; Milan Chromek - [email protected]; Annelie Brauner - [email protected]; Gunvor Ekman-Ordeberg - [email protected] * Corresponding author
Published: 25 August 2005 Reproductive Biology and Endocrinology 2005, 3:39 39
doi:10.1186/1477-7827-3-
Received: 20 June 2005 Accepted: 25 August 2005
This article is available from: http://www.rbej.com/content/3/1/39 © 2005 Törnblom et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract Background: Human cervical ripening is an inflammatory process. In labour at term the mRNAlevels and protein concentrations for interleukin-6 (IL-6) and IL-8 in cervix significantly increase. The aim of this study was to investigate if there are differences in the inflammatory process of preterm and term cervical ripening. Methods: Cervical biopsies from 50 singleton pregnant women without clinical signs of infection were allocated to four groups: preterm labour, term labour, preterm not in labour and term not in labour. The protein levels of IL-8, IL-6, monocyte chemotactic protein-1 (MCP-1), regulated upon activation normal t cells expressed and secreted (RANTES) and tumour necrosis factor-alpha (TNF-alpha) were quantified in tissue homogenates by ELISA or Immulite. The mRNA expression of IL-8, MCP-1 and RANTES was studied using RT-PCR. White blood cell count (WBC) and Creactive protein (CRP) in the blood were determined. For determination of statistically significant differences between study groups Mann-Whitney U test or Kruskal-Wallis test were applied. Results: Protein concentrations of IL-8, IL-6, and MCP-1 were significantly increased during labour compared to non-labouring groups, whereas no changes were observed for RANTES and TNFalpha. The mRNA levels of representative cytokines such as IL-8 and MCP-1 increased significantly during labour whereas RANTES mRNA expression remained unchanged. WBC and CRP were significantly higher in the labouring groups as compared to groups not in labour. For neither of the analysed cytokines, WBC or CR
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