mRNA expression and localization of bNOS, eNOS and iNOS in human cervix at preterm and term labour
- PDF / 1,162,487 Bytes
- 9 Pages / 610 x 792 pts Page_size
- 58 Downloads / 183 Views
BioMed Central
Open Access
Research
mRNA expression and localization of bNOS, eNOS and iNOS in human cervix at preterm and term labour Susanne Abelin Törnblom1, Holger Maul2,3, Aurelija Klimaviciute*1, Robert E Garfield2, Birgitta Byström1, Anders Malmström4 and Gunvor Ekman-Ordeberg1 Address: 1Department of Woman and Child Health, Division of Obstetrics and Gynaecology, Karolinska University Hospital, Karolinska Institute, 171 76 Stockholm, Sweden, 2Department of Obstetrics and Gynaecology, Division of Reproductive Sciences, University of Texas Medical Branch, Galveston, Texas, TX 77555-1062, USA, 3Department of Obstetrics and Gynaecology, University of Heidelberg, 69115 Heidelberg, Germany and 4Dept of Experimental Medical Science, BMC, University of Lund, 221 84 Lund, Sweden Email: Susanne Abelin Törnblom - [email protected]; Holger Maul - [email protected]; Aurelija Klimaviciute* - [email protected]; Robert E Garfield - [email protected]; Birgitta Byström - [email protected]; Anders Malmström - [email protected]; Gunvor Ekman-Ordeberg - [email protected] * Corresponding author
Published: 10 August 2005 Reproductive Biology and Endocrinology 2005, 3:33 33
doi:10.1186/1477-7827-3-
Received: 20 June 2005 Accepted: 10 August 2005
This article is available from: http://www.rbej.com/content/3/1/33 © 2005 Törnblom et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract Background: Preterm birth is the primary cause of the neonatal mortality and morbidity. There will be no preterm birth without a cervical softening. Nitric oxide (NO) is shown to be a mediator of term cervical ripening. The aim of this study was to investigate mRNA expression of the three isomers of NO synthases (NOS) and to identify them by immunohistochemistry in the human cervix at preterm birth compared to term. Methods: The three isomers of NOS- inducible (iNOS), endothelial (eNOS) and neuronal (bNOS) – were investigated in the human cervix. The expression of mRNA was determined using RealTime Multiplex RT-PCR. The localisation of synthases in the cervical tissue was analysed using immunohistochemistry. Cervical biopsies were obtained from 4 groups of women without clinical signs of infection: preterm (PTL), term labour (TL), preterm not in labour (PTnotL) and term not in labour (TnotL) patients. One-Way ANOVA, Kruskal-Wallis, Student t-test or Mann-Whitney test were applied as appropriate to determine statistically significant differences among the groups. Results: Patients in preterm labour had significantly (p < 0.01) higher mRNA levels of all the three NOS isomers compared to those in term labour. Women not in labour, irrespective of gestational age, thus with unripe cervices, had significantly lower eNOS mRNA levels compar
Data Loading...
