Oligo( p -phenylenevinylene)-rhodium complex as intracellular catalyst for enhancing biosynthesis of polyhydroxybutyrate
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igo(p-phenylenevinylene)-rhodium complex as intracellular catalyst for enhancing biosynthesis of polyhydroxybutyrate biomaterials 1,2
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Nan Dai , Hao Zhao , Wen Yu , Fengting Lv , Libing Liu 1
1,2*
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& Shu Wang
Bejing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, 2
Beijing 100190, China; College of Chemistry, University of Chinese Academy of Sciences. Beijing 100049, China Received June 16, 2020; accepted July 18, 2020; published online September 28, 2020
Microbial synthesis utilizes sustainable resources to produce valuable chemicals, as a potential alternative to petroleum-based chemical industry. Although metabolic engineering is an efficient method to enhance the biosynthesis efficacy of microorganisms, it requires complicated biological procedures. Herein, we report a facile intracellular catalysis system for augmenting the production of bio-based material in microorganism. Covalent linking of oligo(p-phenylenevinylene) (OPV) and cyclopentadienyl rhodium(III) bipyridine offers intracellular metal catalyst (OPV-Rh). The OPV-Rh complex displayed certain resistance to toxic biomolecules, which guaranteed its catalytic activity in complicated biological systems. With uptake by Gramnegative bacterium Ralstonia eutropha H16 (R. eutropha H16), the OPV-Rh complex promotes the transformation of in+ tracellular NADP to NADPH, which further enhances the biosynthesis of polyhydroxybutyrate (PHB) by this microorganism. This work demonstrates that synthetic metal catalyst can be employed for regulating microbial biosynthesis intracellularly. microbial synthesis, rhodium complex, NADPH, PHB Citation:
Dai N, Zhao H, Yu W, Lv F, Liu L, Wang S. Oligo(p-phenylenevinylene)-rhodium complex as intracellular catalyst for enhancing biosynthesis of polyhydroxybutyrate biomaterials. Sci China Chem, 2020, 63, https://doi.org/10.1007/s11426-020-9833-8
1 Introduction Intracellular in situ catalysis with synthetic metal catalysts has emerged as an attractive way for modulating functions of living organisms [1–3]. Up to now, researchers have successfully developed many catalytic systems inside cells, including illuminating cells through deprotection reaction [4], inhibiting the tumor cell growth through carbon-carbon cross coupling reaction [5,6], regulating intracellular redox state through catalytic oxidation or reduction of biomolecules [7,8], etc. Despite of the success in realizing catalytic reactions inside living cells, concerns still exist in this field. One of the major challenges lies in the design of suitable metal *Corresponding authors (email: [email protected]; [email protected])
catalysts for transformations in physiological conditions [9]. To play a role in biological system, the catalysts should be stable, water soluble and biocompatible. Another important issue is exploring more biological functions for these catalytic systems rather than just realizing the catalytic conversion of certain molecules [10]. Microbial
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