Optimization of microbiological plastic film test plate conditions for rapid detection of antibiotics in milk
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ORIGINAL PAPER
Optimization of microbiological plastic film test plate conditions for rapid detection of antibiotics in milk Moeketsi Ntakatsane1,3 · Ping Chen1 · Jingsheng Liu1 · Poloko Mosebi3 · Linlin Xu1 · Katleho Senoko2,3 Received: 28 May 2020 / Accepted: 21 July 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Food safety and quality are issues of great concern to food producers and consumers. Key challenges towards achieving these include availability of rapid, user-friendly, economic and reliable techniques to detect problems such as antibiotic residues. Therefore, the present study was conducted to evaluate performance of plastic film test plate (PFTP) as influenced by antibiotic type, test bacterium, inoculum volume and concentration. Comparison was made to the microtiter test plate (MTP) and the National Standard method (SN/T 3979-2014). For this, antibiotic susceptibility testing was conducted on four bacterial types (Micrococcus luteus, Streptococcus thermophillus suspensions, Staphylococcus aureus and Coliform bacteria) against three antibiotics (penicillin G, sulfadiazine and tetracycline). Results showed high susceptibility of Micrococcus luteus to penicillin G with minimum inhibitory concentration of 3 µg L−1 and 1 µg L−1 via PFTP and MTP respectively. Optimum performance was realized at bacterial concentration of 1 04 CFU ml−1 with detection limit of 1 µg L −1, sensitivity and predictive positive value (PPV) of 81–83% and 97–100% respectively. Detection time was recorded as 6 and 9 h for MTP and PFTP respectively compared to the 18–24 h of the National Standard method (SN/T 3979–2014). The microbiological plastic film test plate under optimized bacterial culture conditions demonstrated tremendous potential for rapid and reliable detection of antibiotics in milk. Keywords Plastic film test plate · Bacterial culture · Antibiotic detection · Milk
Introduction Detection of antibiotic residues along the food supply chain is an issue of great concern not only to food producers but also food regulatory bodies in most developing countries. Antibiotics are most commonly used for treatment of livestock diseases such as mastitis. Excessive use of these veterinary drugs without observation of withdrawal periods can result in important economic losses in production of fermented dairy products such as cheese and yoghurt [1, 2]. Furthermore, consumers are exposed to major health risks including allergic reactions, interference with gut microbiota * Ping Chen [email protected] 1
College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, Jilin, China
2
School of Life Sciences, Jilin Agricultural University, Changchun 130118, Jilin, China
3
Faculty of Agriculture, National University of Lesotho, Roma 180, Maseru, Lesotho
and development of antibiotic resistance which can cause failure of antibiotic therapy [3, 4]. Moreover, health problems such as carcinogenicity, mutagenicity, reproductive disorders and chemical poisoning
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