Oxidative stress exacerbates dextran sulfate sodium-induced ulcerative colitis in ICR mice
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ORIGINAL ARTICLE
Oxidative stress exacerbates dextran sulfate sodium-induced ulcerative colitis in ICR mice Nitima Tatiya-aphiradee 1,2 & Waranya Chatuphonprasert 2,3 & Kanokwan Jarukamjorn 1,2 Received: 21 December 2019 / Accepted: 20 May 2020 # Institute of Molecular Biology, Slovak Academy of Sciences 2020
Abstract Ulcerative colitis (UC) is a complex, multifactorial disorder which can be aggravated by oxidative stress. Dextran sulfate sodium (DSS)-induced UC in inbred mice is the most commonly used animal-model. However, these populations lack genetic variability therefore this study aimed to establish a DSS-induced UC model in outbred ICR mice and to assess its association with the oxidant-antioxidant system. Male ICR mice were administered a 40 kDa-DSS solution (6 g/kg/day; 0.25 mL 21% DSS 4 times per day) intragastrically for 4 and 7 consecutive days (n = 5). Disease activity index (DAI) was determined daily from body weight and stool characteristics. At the end of the study, colons were collected to examine histology, myeloperoxidase activity, and expression of superoxide dismutase 1 and 2 (Sod1 and Sod2) and catalase (Cat). DSS-treated mice demonstrated daily increases in DAI score with significantly shortened colons, an indirect marker of inflammation in UC. Histological examination of DSS-treated colons revealed goblet cell loss, crypt damage, and epithelial erosion, followed by infiltration of mast cells to the mucosa with mucin depletion. Myeloperoxidase activity was elevated in the DSS-treated mice while Sod1, Sod2, and Cat were suppressed compared to controls. A DSS-induced UC model was established in ICR mice with associated oxidative stress triggering. This outbred-mouse model could prove useful for investigating the underlying pathophysiological mechanisms of UC in terms of oxidant-antioxidant balance. Keywords Catalase . Dextran sulfate sodium . Disease activity index . Outbred mouse strain . Superoxide dismutase . Ulcerative colitis
Abbreviations CAT catalase DSS dextran sulfate sodium DAI disease activity index IBD inflammatory bowel disease MPO myeloperoxidas ROS reactive oxygen species SOD superoxide dismutase UC ulcerative colitis * Kanokwan Jarukamjorn [email protected] 1
Faculty of Pharmaceutical Sciences, Khon Kaen University, Mitraparb road, Khon Kaen 40002, Thailand
2
Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology (PANPB), Khon Kaen University, 40002 Khon Kaen, Thailand
3
Faculty of Medicine, Mahasarakham University, Mahasarakham 44000, Thailand
Introduction Ulcerative colitis (UC) is a subcategory of inflammatory bowel disease (IBD). It is a disorder in the gastrointestinal system affecting the caecum, colon, and rectum usually in a continuous pattern (Baumgart and Sandborn 2007) and characteristically affecting the mucosal surface of the colon. The lesion initiates in the rectum and extends proximally through the colon. Symptoms of UC are often insidious with gradual onset. Although the precise etiology of UC remains uncle
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