Phosphate fertiliser alters carboxylates and bacterial communities in sweet potato ( Ipomoea batatas (L.) Lam.) rhizoshe
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Phosphate fertiliser alters carboxylates and bacterial communities in sweet potato (Ipomoea batatas (L.) Lam.) rhizosheaths David Minemba & Belinda C. Martin & Megan H. Ryan & Erik J. Veneklaas & Deirdre B. Gleeson
Received: 5 February 2020 / Accepted: 22 July 2020 # Springer Nature Switzerland AG 2020
Abstract Aims This study aimed to determine the structure of the bacterial community inhabiting the roots and rhizosheath of sweet potato cultivars, and how these bacterial communities respond to P addition and subsequent changes in carboxylate exudation by sweet potato roots. Methods Five sweet potato cultivars were grown with and without P addition in a low P soil in a glasshouse: Beauregard and Northern Star (both international cultivars) and Whagi Besta, Maraso and Marasunda which originate from Papua New Guinea. Rhizosheath carboxylates were measured and the root/rhizosheath bacterial community analysed using 16S rRNA gene sequencing.
Results Carboxylate amount per g root weight was reduced (citrate) or increased (malate and oxalate) with addition of P. Carboxylate amount also positively correlated (citrate, oxalate and shikimate) or negatively correlated (malate) to bacterial alpha diversity when no P was added. P addition decreased bacterial diversity, altered the structure of the bacterial community (taxa and predicted function) and led to an enrichment of bacteria related to known P-solubilising bacteria (Burkholderia and Massilia) as well as to functions related to biosynthesis. Conclusion This study provides a first indication of types and putative roles of root/rhizosheath bacteria in sweet potato cultivars originating from a wide range of environments including marginal and P-impoverished soils and high-P agricultural systems.
Responsible Editor: Tim S. George Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11104-020-04646-6) contains supplementary material, which is available to authorized users. D. Minemba : M. H. Ryan : E. J. Veneklaas : D. B. Gleeson (*) UWA School of Agriculture and Environment and Institute of Agriculture, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia e-mail: [email protected] B. C. Martin : E. J. Veneklaas School of Biological Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia B. C. Martin Ooid Scientific, 98 Edmund Street, White Gum Valley, WA 6162, Australia
Keywords 16S rRNA amplicon sequencing . Rhizosphere . Burkholderia . Papua New Guinea (PNG) . PICRUSt2
Introduction Sweet potato (Ipomoea batatas (L) Lam.) is the sixth most important food crop globally, with production of 100 million tonnes annually (FAO 2016). It is widely recognised as a cheap, nutritious food source in developing countries such as Papua New Guinea (PNG) where it is an essential subsistence food crop with a large economic and social importance (Bourke and Harwood 2009). Currently, 80–85% of the global
Plant Soil
supply of sweet potato is produced by China, w
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