Phospholipids of Embryogenic and Non-embryogenic Cell Lines of Larix sibirica Ledeb .
- PDF / 353,828 Bytes
- 7 Pages / 612 x 792 pts (letter) Page_size
- 87 Downloads / 177 Views
ARCH PAPERS
Phospholipids of Embryogenic and Non-embryogenic Cell Lines of Larix sibirica Ledeb. N. V. Semenovaa, *, V. N. Shmakova, Yu. M. Konstantinova, and L. V. Dudarevaa aSiberian Institute of Plant Physiology and Biochemistry, Siberian Branch, Russian Academy of Sciences, Irkutsk, 664033 Russia
*e-mail: [email protected] Received February 14, 2020; revised April 16, 2020; accepted April 16, 2020
Abstract—Composition of phospholipids was examined in embryogenic and non-embryogenic cell lines of Siberian larch (Larix sibirica Ledeb.). Significant differences in the content and composition of phospholipids were revealed between the cell lines. Phosphatidylcholines (PC) and phosphatidylethanolamines (PE) were predominant phospholipids in embryogenic cell lines, whereas non-embryogenic cell lines featured a high content of phosphatidic acid (>64% of total phospholipids). The PC/PE ratio known to characterize the membrane structure and function was 1.4–3.8 times higher for embryogenic cell lines than for non-embryogenic lines; this was probably caused by the involvement of these phospholipids in tissue differentiation. The content of phosphatidylinositols differed significantly for embryogenic and non-embryogenic cell lines, where it equaled to 0.50–1.06 and 0.17–0.34 mg/g dry wt, respectively. The observed variations in the composition of phospholipids between the cell lines with different embryogenic potential provide evidence for the important role of these lipids in embryogenesis at the stage of embryo formation. Keywords: Larix sibirica, somatic embryogenesis, cell lines, phospholipids DOI: 10.1134/S1021443720060151
INTRODUCTION A promising direction in biotechnology of microclonal propagation of woody plants in vitro is somatic embryogenesis that allows massive production of plants identical to the maternal genotype by virtue of somatic plant cells [1]. The formation of somatic embryos of most coniferous species, including Siberian larch, remains a challenge, primarily because of scarce knowledge on the conditions and mechanisms of somatic embryogenesis induction in gymnosperms [2]. In particular, the role of lipid components in the induction and development of conifer embryogenesis under in vitro culturing remains poorly understood. There are only few works dedicated to this problem [3, 4]. On the whole, the lipid metabolism of coniferous plants is not yet fully clarified, in particular the composition and content of lipids in tissue cultures of species from the division Pinophyta. The lipid composition of cells and tissues is known to depend on their physiological conditions. Furthermore, the content and composition of lipids in all organelles is subject to interspecific and intraspecific variations and undergoes alterations during plant Abbreviations: CL—cell line; DPG—diphosphatidylglycerols; PA—phosphatidic acid; PC—phosphatidylcholines; PE—phosphatidylethanolamines; PG—phosphatidylglycerols; PI—phosphatidylinositols; PLun—unidentified phospholipids; PPI—polyphosphoinositols; PS—phosphatidylserine
Data Loading...
