Puccinia melanocephala and Salt Stress Modified the Inositol Phosphate and TOR Signalling Pathway in Sugarcane
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RESEARCH ARTICLE
Puccinia melanocephala and Salt Stress Modified the Inositol Phosphate and TOR Signalling Pathway in Sugarcane Marı´a I. Oloriz1,2 • Orelvis Portal3,4 • Luis Rojas1 • Vı´ctor Gil4 • Ba´rbara Ocan˜a1 Naylin Machado1,3,4 • Marilin Herna´ndez1 • Elio Jime´nez1,5 • Monica Ho¨fte2
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Received: 9 October 2019 / Accepted: 9 September 2020 Ó Society for Sugar Research & Promotion 2020
Abstract Sugarcane (Saccharum spp. hybrids) is the primary source of sugar and renewable biofuel energy worldwide. Nevertheless, sugarcane production is affected by different biotic and abiotic stresses. In this work, we analysed the impact of Puccinia melanocephala infection and salt stress on the expression profile of genes related with inositol phosphate synthesis and TOR (Target of Rapamycin) signalling pathway regulation in sugarcane. In this sense, two-month-old plants of rust resistant and susceptible cultivars were infected with P. melanocephala and leaf samples were collected daily during the first seven days post-inoculation. A second group of plants was treated with NaCl, previous to leaf sampled at 2 h and 72 h poststress, leaf chlorophyll content was measured. Quantitative PCR was used to obtain gene expression profiles. Tctp gene was up-regulated in the rust resistant cultivar suggesting
& Marı´a I. Oloriz [email protected] 1
Instituto de Biotecnologı´a de las Plantas, Universidad Central ‘‘Marta Abreu’’ de Las Villas, Carretera a Camajuanı´ km 5.5, 54830 Santa Clara, Cuba
2
Department of Plants and Crops, Laboratory of Phytopathology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Ghent, Belgium
3
Departamento de Biologı´a, Facultad de Ciencias Agropecuarias, Universidad Central ‘‘Marta Abreu’’ de Las Villas, Carretera a Camajuanı´ km 5.5, 54830 Santa Clara, Cuba
4
Centro de Investigaciones Agropecuarias, Facultad de Ciencias Agropecuarias, Universidad Central ‘‘Marta Abreu’’ de Las Villas, Carretera a Camajuanı´ km 5.5, 54830 Santa Clara, Cuba
5
Present Address: Florida Crystals Corp., P.O. Box 86, 21250 US Highway 27 South, South Bay, FL 33493, USA
that a component of TOR signalling pathway is involving in early pathogen recognition. Biotic and abiotic stresses modified the transcript profile of Mips2 and Tap46 genes, depending on the sugarcane stress resistance. Our results contribute to a better understanding of stress signalling networks in sugarcane. Keywords Gene expression Myo-inositol-1-phosphate synthase Puccinia melanocephala Salt stress Translationally controlled tumour protein TAP46 Abbreviations ACT Actin DPI Days postinoculation HR Hypersensitive response MIPS Myo-inositol-1-phosphate synthase TAP46 Protein phosphatase2A (PP2A)-associated protein TAP46L Protein phosphatase2A (PP2A)-associated protein (leaf) TCTP Translationally controlled tumour protein TOR Target of Rapamycin
Introduction Sugarcane is an important crop cultivated throughout the tropical and subtropical regions around the world. Biotic and abiotic stresses are the main current
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