Quantitative phosphoproteome on the silkworm ( Bombyx mori ) cells infected with baculovirus
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RESEARCH
Open Access
Quantitative phosphoproteome on the silkworm (Bombyx mori) cells infected with baculovirus Jauharotus Shobahah1,2, Shengjie Xue1,2, Dongbing Hu1,2, Cui Zhao1,2, Ming Wei1,2, Yanping Quan1,2 and Wei Yu1,2*
Abstract Background: Bombyx mori has become an important model organism for many fundamental studies. Bombyx mori nucleopolyhedrovirus (BmNPV) is a significant pathogen to Bombyx mori, yet also an efficient vector for recombinant protein production. A previous study indicated that acetylation plays many vital roles in several cellular processes of Bombyx mori while global phosphorylation pattern upon BmNPV infection remains elusive. Method: Employing tandem mass tag (TMT) labeling and phosphorylation affinity enrichment followed by highresolution LC-MS/MS analysis and intensive bioinformatics analysis, the quantitative phosphoproteome in Bombyx mori cells infected by BmNPV at 24 hpi with an MOI of 10 was extensively examined. Results: Totally, 6480 phosphorylation sites in 2112 protein groups were identified, among which 4764 sites in 1717 proteins were quantified. Among the quantified proteins, 81 up-regulated and 25 down-regulated sites were identified with significant criteria (the quantitative ratio above 1.3 was considered as up-regulation and below 0.77 was considered as down-regulation) and with significant p-value (p < 0.05). Some proteins of BmNPV were also hyperphosphorylated during infection, such as P6.9, 39 K, LEF-6, Ac58-like protein, Ac82-like protein and BRO-D. Conclusion: The phosphorylated proteins were primary involved in several specific functions, out of which, we focused on the binding activity, protein synthesis, viral replication and apoptosis through kinase activity. Keywords: Bombyx mori, BmNPV, Phosphorylation, Proteomic, Tandem mass tag, Regulation
Background Bombyx mori, the domesticated silkworm, is an economically significant insect in silk production [1]. Farmers in many developing countries such as China, India, Brazil, Vietnam, and Thailand are evolving sericulture as a foremost income source. China, the biggest cocoon producer (almost 80%) worldwide, generated a sericulture-based income of 22.4 billion ¥ (Yuan) (approximately equal to USD 3.24 billion by March 2017) by producing 6.61 × 108 kg of cocoons in 2011 [2]. Moreover, this lepidopteran insect has become a model organism for several fundamental studies in biochemistry, molecular genetics, and genomics [3, 4]. * Correspondence: [email protected] 1 Institute of Biochemistry, College of Life Sciences, Zhejiang Sci-Tech University, Xiasha High-Tech Zone No.2 RoadZhejiang Province, Hangzhou 310018, People’s Republic of China 2 Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Zhejiang Province, Hangzhou 310018, People’s Republic of China
In-depth studies of silkworm were extensively carried out since its genome was completely sequenced in 2004 [1]. H3 N-terminus, an N-terminal tail of histone H3 in holocentric chromosomes of the silkworm, is reported to be extensively acetylated a
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