Response of the gut microbiota during the Clostridioides difficile infection in tree shrews mimics those in humans
- PDF / 3,297,593 Bytes
- 13 Pages / 595.276 x 790.866 pts Page_size
- 33 Downloads / 210 Views
RESEARCH ARTICLE
Open Access
Response of the gut microbiota during the Clostridioides difficile infection in tree shrews mimics those in humans Wenpeng Gu1,2, Wenge Li3, Wenguang Wang1, Dexuan Kuang1, Wenzhu Zhang3, Caixia Lu1, Na Li1, Pinfen Tong1, Yuanyuan Han1, Xiaomei Sun1, Jinxing Lu3, Yuan Wu3* and Jiejie Dai1*
Abstract Background: Clostridioides difficile is a major cause of antibiotic associated diarrhea. Several animal models are used to study C. difficile infection (CDI). The tree shrew has recently been developed as a model of primate processes. C. difficile infection has not been examined in tree shrews. We infected tree shrews with hyper-virulent C. difficile strains and examined the alterations in gut microbiota using 16S rRNA gene sequencing. Results: C. difficile colonized the gastrointestinal tract of tree shrew and caused diarrhea and weight loss. Histopathologic examination indicated structures and mucosal cell destruction in ileal and colonic tissues. The gut microbial community was highly diversity before infection and was dominated by Firmicutes, Fusobacteria, Bacteroidetes, and Proteobacteria. Antibiotic administration decreased the diversity of the gut microbiota and led to an outgrowth of Lactobacillus. The relative abundance of Proteobacteria, Gammaproteobacteria, Enterobacteriales, Lachnospiraceae, Enterobacteriaceae, Escherichia, Blautia, and Tyzzerella increased following C. difficile infection. These taxa could be biomarkers for C. difficile colonization. Conclusions: In general, the disease symptoms, histopathology, and gut microbiota changes following C. difficile infection in tree shrews were similar to those observed in humans. Keywords: Clostridioides difficile, Tree shrew, Gut microbiota
Background Clostridioides difficile is a major cause of antibiotic associated diarrhea in patients after hospitalization and antibiotic administration [1]. C. difficile infection (CDI) caused by toxigenic isolates can result in a wide range of * Correspondence: [email protected]; [email protected] 3 State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Chang bai Road 155, Chang ping District, Beijing 102206, China 1 Center of Tree Shrew Germplasm Resources, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases, Yunnan Innovation Team of Standardization and Application Research in Tree Shrew, Zhao zong Road 66, Kunming 650118, China Full list of author information is available at the end of the article
outcomes, including asymptomatic colonization, diarrhea, life threatening pseudomembranous colitis, or intestinal obstruction [2]. CDI is the most common cause of healthcare-associated diarrhea and colitis, and is responsible for significant morbidity a
Data Loading...
