RETRACTED ARTICLE: The microRNA-1246 promotes metastasis in non-small cell lung cancer by targeting cytoplasmic polyaden
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RESEARCH
Open Access
The microRNA-1246 promotes metastasis in non-small cell lung cancer by targeting cytoplasmic polyadenylation element-binding protein 4 Weihua Huang1, Huifen Li2 and Rongcheng Luo1*
Abstract Background: The microRNAs present a class of non-coding RNAs which are usually implicated in tumor biology. Recent report has unraveled that a novel member of microRNA family called miR-1246. However, the functional role and molecular mechanisms of miR-1246 in non-small cell lung cancer (NSCLC) is still elusive. Methods: Using RT-PCR, luciferase reporter, mRNA microarrays, invasion and migration assays, we investigated the potential role of miR-1246 in the pathogenesis of NSCLC. Results: In this study, we showed that miR-1246 markedly promoted NSCLC cell migration and invasion. Meanwhile, we found that cytoplasmic polyadenylation element binding protein 4 (CPEB4) might be involved and serve as a direct target of miR-1246 in NSCLC. CPEB4 knockdown substantially enhanced NSCLC migration and invasion resembling the effect of miR-1246 in NSCLC. CPEB4 is also frequently downregulated in NSCLC and decreased CPEB4 expression correlated with poor survival. Conclusions: These results suggested that the miR-1246 may promote cell metastasis by targeting CPEB4. Meanwhile, the level of CPEB4 could be used as a potential marker in NSCLC patients. Our findings unraveled novel functions of miR-1246 in lung cancer cells and shed light on NSCLC prognosis. Keywords: miR-1246, CPEB4, NSCLC, Metastasis
Background Lung cancer is the leading cause of cancer-related mortality worldwide [1]. Lung cancer has been traditionally subdivided into two principal groups, namely, neuroendocrine and non-small cell lung cancer (NSCLC). The latter type is more common than the former. Cancer occurs and develops as a complicated result of an accumulation of various endogenous and exogenous effects. Gene alterations participate in cancer genesis. Alterations in many onco3 genes and tumor suppressor genes have been reported in lung cancer. MicroRNAs (miRNA) are small noncoding RNA molecules that primarily serve as a posttranscriptional factor * Correspondence: [email protected] 1 TCM-Integrated Hospital, Southern Medical University, Cancer Center, NO.13 Shiliugang Road, Haizhu District, Guangzhou, Guangdong 510315, China Full list of author information is available at the end of the article
for gene expression. The miRNA can also function by base-pairing with the 3’-untranslated region (3’-UTR) of specific mRNAs [2]. Aberrant expression of miRNAs are often regarded as biomarkers of biological pathways leading to the occurrence of malignancy including cancer [3]. Many recent reports have clarified critical roles for miRNAs in regulating tumor cell invasion, metastasis and migration [4]. It is well known that miRNAs can participate in numerous biological processes, such as apoptosis, differentiation and invasion. During tumorigenesis, miRNAs may act either as an oncogene or a tumor suppressor and contribute to tumor initiation and progr
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