Saccharothrix algeriensis NRRL B-24137, the first non- Streptomyces actinobacterium, produces holomycin after cystine fe
- PDF / 968,787 Bytes
- 8 Pages / 595.276 x 790.866 pts Page_size
- 58 Downloads / 133 Views
ORIGINAL PAPER
Saccharothrix algeriensis NRRL B‑24137, the first non‑Streptomyces actinobacterium, produces holomycin after cystine feeding Rabiâa Merrouche1 · Amine Yekkour1,2 · Yannick Coppel3 · Noureddine Bouras1,4 · Abdelghani Zitouni1 · Florence Mathieu5 · Nasserdine Sabaou1 Received: 2 November 2019 / Revised: 28 June 2020 / Accepted: 30 June 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Saccharothrix algeriensis NRRL B-24137 is an actinobacterium isolated from Algerian Saharan soil. This strain has the ability to produce several dithiolopyrrolone antibiotic derivatives depending on the precursors added to the culture medium. This group of antibiotics is known for their potent antimicrobial and anticancer activities. Holomycin is a member of the dithiolopyrrolone group of antibiotics, and has already been isolated from several species of actinobacteria belonging to the genus Streptomyces and also from some Gram-negative bacteria. In this study, holomycin was produced for the first time in the culture broth of a non-Streptomyces actinobacteria. This antibiotic was induced by adding 5 mM of L-cystine as precursor to the semi-synthetic fermentation broth of Sa. algeriensis NRRL B-24137 and then fully identified after HPLC purification. The minimum inhibitory concentrations (MIC) of holomycin were determined against several pathogenic microorganisms, including Escherichia coli ATCC 10536 Klebsiella pneumoniae CIP 82.91, Listeria monocytogenes CIP 82110, Staphylococcus aureus CIP 7625, Aspergillus carbonarius M333, Fusarium culmorum FC1, Candida albicans IPA 200. This antibiotic showed a broad-spectrum antimicrobial activity, inhibiting a variety of Gram-positive and Gram-negative bacteria, and micro-fungi. Keywords Dithiolopyrrolone antibiotics · Holomycin · Saccharothrix algeriensis NRRL B-24137 · L-cystine · Antimicrobial activity
Introduction Communicated by Erko Stackebrandt. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-01971-3) contains supplementary material, which is available to authorized users. * Amine Yekkour [email protected] 1
Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria
2
Institut National de Recherche Agronomique d’Algérie, Station Mehdi Boualem-Baraki, Alger, Algeria
3
Laboratoire de Chimie de Coordination, CNRS, Université de Toulouse, UPS, INPT, Toulouse, France
4
Département de Biologie, Faculté des Sciences de la Nature et de la Vie et Sciences de la Terre, Université de Ghardaia, Ghardaïa, Algeria
5
Laboratoire de Génie Chimique, Université de Toulouse, CNRS, Toulouse, France
Dithiolopyrrolones, which possess a unique pyrrolinonodithiole skeleton linked to two variable acyl groups, are a group of natural antibiotics that have showed strong broad-spectrum activities towards various Gram-positive and Gram-negative bacteria, protozoa, yeast, pathogenic micro-fungi, and also against different cell lines invo
Data Loading...
