Streptomyces sp. S501, a Marine Petroleum-Degrading Actinobacterium Isolated from Sediment of Yalujiang Estuary, Norther
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Streptomyces sp. S501, a Marine Petroleum‑Degrading Actinobacterium Isolated from Sediment of Yalujiang Estuary, Northern China, and Its Genome Annotation Chao Chen1 · Shuai Chen1 · Wanxing Zhang1 · Fenghao Yuan1 · Jicheng Yu1 · Qiu Liu1 Received: 3 September 2019 / Accepted: 25 August 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Streptomyces sp. S501, which was isolated from the sediment of Yalujiang Estuary in China, was the first marine Streptomyces species discovered to act as an excellent petroleum degrader. We analyzed the effect of pH, temperature, and concentration of NH4NO3 on the petroleum degradation of strain S501, and the optimum biodegradation rate reached 63.02% under the condition of 2 g/L N H4NO3 addition at 30 °C and pH 8. The complete genome sequence of Streptomyces sp. S501 was determined by using the PacBio RSII platform, which contains a linear chromosome with 7,173,651 bp and a linear plasmid with 288,181 bp, with GC contents of 71.19% and 67.57%, respectively. The genome sequence suggests that Streptomyces sp. S501 has the ability to degrade several hazardous pollutants, as well as the ability to biosynthesize diverse secondary metabolites and enzymes. There are fifty annotated genes involved in oil component degradation, and there are three genes without known annotation information in Streptomyces sp. S501, which have high homology with genes encoding P450 family enzymes and should be novel genes involved in alkane degradation. This study provides useful genetic information for investigating the molecular mechanisms of marine Streptomyces, with biodegradation and application potential.
Introduction Petroleum is perhaps the most complex organic compound on earth, and because of natural and anthropogenic sources, a large amount of petroleum enters the marine environment and destroys the balance of the ecosystem [1]. Microorganisms play key roles in biogeochemical processes in ocean environments, which are marine inhabitants and respond to environmental changes rapidly [2]. Petroleum pollution in the ocean enriches microorganisms that can degrade petroleum hydrocarbons, and researchers have tried to determine the roles of different kinds of bacteria during the Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00284-020-02181-2) contains supplementary material, which is available to authorized users. * Jicheng Yu [email protected] * Qiu Liu [email protected] 1
Institute of Marine Microbiology, College of Life Science, Dalian Minzu University, Dalian 116600, Liaoning, People’s Republic of China
biodegradation of petroleum hydrocarbons [3, 4]. There are many marine bacteria certified to be good biodegraders of petroleum hydrocarbons [1], but for marine Streptomyces spp., there remain many unknowns. Researchers have found that Streptomyces isolated from soil and plants are powerful biotechnological tools for the biodegradation of hazardous materials that contaminate human-inhabited environments [5].
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