Screen the Effective Components of Lycopodii herba on Rheumatoid Arthritis with the Aid of Spectrum-Effect Relationship
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ORIGINAL ARTICLE
Screen the Effective Components of Lycopodii herba on Rheumatoid Arthritis with the Aid of Spectrum-Effect Relationship and Uncover its Potential Mechanism Zhen Yang,1 Qingsheng Yin,1 Jing Ma,1 Changshuo Yang,1 Yuanyuan Sheng,1 Lili Song,1 Tan Pang,1 Pengwei Zhuang,1,2 Hong Guo,1,3 and Yanjun Zhang 1,2,3
Lycopodii herba (SJC), a traditional Chinese medicine, has the effect of dispelling wind and eliminating dampness (a therapeutic principle and method of traditional Chinese medicine for rheumatoid arthritis), relaxing tendon and activating collaterals. However, the major effective components and its therapeutic mechanism were unclear. In this study, different SJC samples with slightly different compositions were prepared by extracting with different concentrations of ethanol. Then, the therapeutic effects on rheumatoid arthritis (RA) of different SJC samples were evaluated. Finally, the spectrum–effect relationship between UPLC-Q-TOF/MS fingerprints and the effect of RA was explored to screen the effective components. Western blotting was used to study the potential mechanism. The volume of hind paw and the level of RF, TNF-α, and IL1β were lower after administrating with different SJC samples, compared with the model group. Histopathological findings also confirmed that SJC could relieve the symptoms of RA. Combined with identification of the components in plasm from SJC, lycojaponicumin C, des-N-methyl-α-obscurine, 8β-acetoxy-12β-hydroxy-lycopodine or 8β-acetoxy11α-hydroxy-lycopodine or 8β-hydroxy-11α-acetoxylycopodine were considered to be the major effective components. The mechanism may be related to AChE/NF-κB signaling pathway. This work provides a general method to screen the potential effective components of herb medicines and would be benefit to understand the mechanism of SJC for the treatment of RA. Abstract—
KEY WORDS: Lycopodii herba; rheumatoid arthritis; spectrum–effect relationship.
1
Chinese Materia Medica College, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China 2 Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China 3 To whom correspondence should be addressed to Hong Guo at Chinese Materia Medica College, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China. E-mail: [email protected]; and ; [email protected]
INTRODUCTION Rheumatoid arthritis (RA) is a debilitating autoimmune disease characterized by symmetrical peripheral polyarthritis and chronic inflammation of the synovial joints that leads to joint destruction and functional limitations [1– 3]. The prevalence of RA is believed to range from 0.4 to
0360-3997/20/0000-0001/0 # 2020 Springer Science+Business Media, LLC, part of Springer Nature
Yang, Yin, Ma, Yang, Sheng, Song, Pang, Zhuang, Guo, and Zhang 1.3% worldwide [4], and its clinical manifestations include pain, swelling, and redness [5]. The pathogenesis is complex and often accompanied with immune response, involving the production of a
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