SERPINE-1 Gene Methylation and Protein as Molecular Predictors of Laparoscopic Sleeve Gastrectomy Outcome
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ORIGINAL CONTRIBUTIONS
SERPINE-1 Gene Methylation and Protein as Molecular Predictors of Laparoscopic Sleeve Gastrectomy Outcome Sara Assem 1 & Tamer N. Abdelbaki 2 & Safaa H. Mohy-El Dine 1 & Amel F. Ketat 1 & Doaa A. Abdelmonsif 1,3
# Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Background Body weight is subjected to genetic and epigenetic modifiers that might affect the success of weight loss interventions. Because of its possible complications and disparity in patients’ response, identification of predictors to the outcome of bariatric surgery is indispensable. Objectives This prospective study aims to investigate serpin peptidase inhibitor type 1 (SERPINE-1) protein and gene methylation as molecular predictors to the outcome of bariatric surgery. Patients and Methods One hundred participants were enrolled and divided to control group (n = 50) and obese patients who underwent laparoscopic sleeve gastrectomy (LSG) (n = 50). Anthropometric measurements were assessed and blood samples were collected preoperatively and 6 months postoperatively for assessment of SERPINE-1 protein and gene methylation, Creactive protein (CRP), and homeostatic model assessment of insulin resistance (HOMA-IR). Moreover, subjects were followed for 2 years for weight loss parameters. Results Patients with obesity showed high baseline SERPINE-1 protein and gene hypermethylation where LSG was followed by a drop in SERPINE-1 protein level but not gene hypermethylation. Baseline SERPINE-1 gene methylation was negatively related to postoperative weight loss and was the independent predictor to weight loss after LSG. Likewise, postoperative SERPINE-1 protein was negatively related to weight loss with independent expression from its gene methylation state. Furthermore, postoperative SERPINE-1 gene methylation correlated to CRP and HOMA-IR. Conclusion Baseline SERPINE-1 gene methylation might be a predictor of weight loss after LSG. Meanwhile, postoperative SERPINE-1 protein could be a predictor to weight loss maintenance after LSG. Lastly, postoperative SERPINE-1 gene methylation might serve as an index to postoperative changes in obesity-related comorbidities. Keywords SERPINE-1 . Gene methylation . LSG . CRP . HOMA-IR
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11695-020-04533-0) contains supplementary material, which is available to authorized users. * Doaa A. Abdelmonsif [email protected]; [email protected] Sara Assem [email protected] Tamer N. Abdelbaki [email protected] Safaa H. Mohy-El Dine [email protected]
Amel F. Ketat [email protected] 1
Department of Medical Biochemistry, Faculty of Medicine, University of Alexandria, Alexandria, Egypt
2
Department of Surgery, Faculty of Medicine, University of Alexandria, Alexandria, Egypt
3
Molecular Biology Lab. and Nanomedicine Lab., Center of Excellence for Research in Regenerative Medicine and Applications, University of Alexandria, Alexandria, Egypt
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