Serum a proliferation-inducing ligand and MicroRNA-223 are associated with rheumatoid arthritis: diagnostic and prognost
- PDF / 730,547 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 0 Downloads / 182 Views
Molecular Medicine
RESEARCH ARTICLE
Open Access
Serum a proliferation-inducing ligand and MicroRNA-223 are associated with rheumatoid arthritis: diagnostic and prognostic implications Mohamed Taha1* , Olfat Gamil Shaker2, Enas Abdelsalam3 and Noha Taha4
Abstract Background: Current blood-based tests for rheumatoid arthritis (RA) have inherent limitations, necessitating the need for additional new biomarkers for its diagnosis and monitoring disease activity and responsiveness to therapy. MicroRNAs (miRNAs) and a proliferation-inducing ligand (APRIL) are deregulated in RA and were linked to its pathogenesis. This study investigated serum levels of APRIL, miR-223 and miR-155 in RA patients, their potential as diagnostic and prognostic biomarkers, and their correlation with disease activity and clinicopathological data. Methods: One hundred and twenty Egyptian patients with RA and 130 healthy controls were included. Serum miRNAs and APRIL were assayed by RT-qPCR and ELISA, respectively. Results: Serum APRIL and miR-223 were significantly upregulated, while miR-155 was unchanged in RA patients compared to controls. Serum miR-223 discriminated RA patients from controls with AUC = 0.85, whereas serum APRIL superiorly distinguished the two groups with AUC = 1 (sensitivity and specificity = 100% at cutoff> 4.19 ng/ml) by receiver-operating-characteristic analysis. Serum miR-223 was a significant predictor for RA diagnosis in multivariate logistic regression analysis. In RA group, serum APRIL was positively correlated with disease activity score (DAS28-CRP). Serum miR-223 expression was positively correlated with serum miR-155, APRIL levels and with the presence of subcutaneous nodules. Serum miR-155 levels were correlated with antinuclear antibody titer in reverse direction. Conclusion: Our results suggest serum APRIL and miR-223 could serve as potential biomarkers of RA, with miR-223 as a predictor of RA risk and APRIL as an excellent biomarker of disease activity. Our data could be implicated for accurate and blood-based non-invasive diagnosis and prognosis of RA. Keywords: APRIL, Autoimmune disease, Cytokine, miRNAs, Rheumatid
Introduction Rheumatoid arthritis (RA) is a debilitating chronic inflammatory condition of the joints that affects approximately 1% of the world’s population (Ogrendik 2013) and about 0.3% of the Egyptian population (Gamal et al. 2016). It is a systemic autoimmune disease characterized * Correspondence: [email protected] 1 Biochemistry Department, Faculty of Pharmacy, Cairo University, 23 Kasr Al Ainy Street, Cairo 11562, Egypt Full list of author information is available at the end of the article
by inflammation and hyperplasia, hypertrophy and angiogenesis of synovial tissues, with cartilage and bone destruction that lead to joint swelling, pain and stiffness (Ogrendik 2013; Gamal et al. 2016). The genesis of RA involves complex multifactorial steps in which an interplay exists between genetic, epigenetic, immunological and environmental factors, but knowledge of the full molec
Data Loading...
