Simultaneous identification to monitor consortia strain dynamics of four biofuel yeast species during fermentation
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BIOTECHNOLOGY METHODS - ORIGINAL PAPER
Simultaneous identification to monitor consortia strain dynamics of four biofuel yeast species during fermentation Gabriel Perez1 · Florencia Debernardis1 · Eduardo Boido1 · Francisco Carrau1 Received: 19 June 2020 / Accepted: 7 September 2020 © Society for Industrial Microbiology and Biotechnology 2020
Abstract Mixed strain dynamics are still not well or easily monitored although recently molecular identification methods have improved our knowledge. This study used a chromogenic differential plating medium that allows the discrimination of four of the main selected biofuel strains that are currently under development for ethanol production from cellulosic hydrolysates. Complete fermentation of hexoses and xylose was obtained with a yeast consortium composed of Spathaspora passalidarum, Scheffersomyces stipitis, Candida akabanensis and Saccharomyces cerevisiae. The results showed that C.akabanensis excessively dominated consortium balance. Reducing its inoculum from 33 to 4.8% improved population strain balance and fermentation efficiency. Comparison of the consortia with single strain fermentations showed that it optimize sugar consumption and ethanol yields. This simple and cheap method also has advantages compared with molecular methods, as the yeast strains do not need to be genetically marked and identified cell proportions are probably active in the fermentation system as compared to DNA determination methods. Keyword Candida akabanensis · Spathaspora passalidarum · Scheffersomyces stipitis · Colony differentiation · Cellobiose
Introduction Renewable biofuels from cellulosic feedstocks could potentially replace a fraction of the fossil fuels used for transportation. The recovery of lignocellulosic residues will be a key source for the development of alternative energies due to its rich sugar components [1]. Lignocellulose is the most abundant component of biomass (mainly wood and agricultural crops), but the challenge to using this resource is the hydrolysis of cellulose and hemicellulose, the main sources of sugars for biofuels, with low cost technologies [2, 3]. Although there are physicochemical methods that allow use of biomass in the production of fuels, a promising cheaper and sustainable alternative is microbial Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10295-020-02310-7) contains supplementary material, which is available to authorized users. * Francisco Carrau [email protected] 1
Área Enología y Biotecnología de la Fermentación, Departamento Ciencia y Tecnología Alimentos, Facultad de Quimica, Universidad de la Republica, 11800 Montevideo, Uruguay
fermentation to ethanol [4, 5]. The ideal and cheap process will be to simultaneously hydrolyse the recalcitrant lignocelluloses composed of lignin which limit accessibility to celluloses and hemicelluloses and subsequent ethanol fermentation of hexoses and pentoses [6]. However, this process will require a controlled sequence of microbial conditions du
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