Study of Interaction between Metallothionein and CdTe Quantum Dots
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ORIGINAL
Study of Interaction between Metallothionein and CdTe Quantum Dots Sylvie Skalickova • Ondrej Zitka • Lukas Nejdl • Sona Krizkova Jiri Sochor • Libor Janu • Marketa Ryvolova • David Hynek • Jarmila Zidkova • Vaclav Zidek • Vojtech Adam • Rene Kizek
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Received: 19 July 2012 / Revised: 15 January 2013 / Accepted: 31 January 2013 / Published online: 16 February 2013 Ó Springer-Verlag Berlin Heidelberg 2013
Abstract Quantum dots (QDs) belong to a new class of fluorescent agent for biochemical, medicinal or other purposes. However, QDs based on cadmium or other metals can be risky for an organism. As one of the mechanism how to detoxify cadmium-based QDs expression of metallothioneins (MT) can be considered. Due to high affinity of metallothionein to cadmium(II) ions, we attempted to develop an approach for studying of possible interaction with QDs. We prepared QDs with CdTe core and studied the interaction with MT, which we isolated from livers of Cd-administered rabbits. To study the interaction, we used the mixture of both components MT (3.6 lM): CdTe QDs (0, 0.34, 0.68, 1.02, 1.36, 1.7, 2.04 and 2.47 lM). The mixtures were studied by spectrophotometry within the range from 200 to 750 nm with detected maxima at 260 and 505 nm. Same mixtures were also
Published in the special paper collection ‘‘Advances in Chromatography and Electrophoresis and Chiranal 2012’’ with guest editor Jan Petr. S. Skalickova O. Zitka L. Nejdl S. Krizkova J. Sochor L. Janu M. Ryvolova D. Hynek V. Adam R. Kizek (&) Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech Republic e-mail: [email protected] O. Zitka S. Krizkova J. Sochor M. Ryvolova D. Hynek V. Adam R. Kizek Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, 616 00 Brno, Czech Republic J. Zidkova V. Zidek Department of Biochemistry and Microbiology, Institute of Chemical Technology, Technicka 3, 166 28 Prague, Czech Republic
analysed by differential pulse voltammetry Brdicka reaction, which supported data from spectrophotometry. Subsequently, we used fast protein liquid chromatography for purification of protein–quantum dot conjugates. We obtained the different chromatograms for (1) Apo MT, (2) CdTe QDs and (3) MT–QD complex. We also collected the fractions and subsequently analysed them on the content of Cd and MT, which confirmed the formation of CdTe QDs– MT complex. Keywords Fast protein liquid chromatography Brdicka reaction Differential pulse voltammetry Fluorimetry Spectrophotometry Separation of quantum dots ApoMT
Introduction Quantum dots (QDs) light-emitting particles on the nanometre scale are emerging as a new class of fluorescent agent for in vivo imaging [1]. QDs often consist of cadmium(II) ions and/or ions of other metal such as selenium, tellurium or zinc [2] and can be used for fluorescent labelling of biomolecules [3, 4]. In addition, these particles can be modified by a recognition molecule such a
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