Study on Biological Effect of Plasticizer
In order to describe the influence of long-term and low dose exposure of plasticizer, the chronic toxicity of Dibutyl phthalate (DBP) to Daphnia magna was determined according to the residual concentration of DBP in the practical environment. The effect o
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Study on Biological Effect of Plasticizer
Yutao Gong, Minling Gao and Chunxiao Sun Abstract In order to describe the influence of long-term and low dose exposure of plasticizer, the chronic toxicity of Dibutyl phthalate (DBP) to Daphnia magna was determined according to the residual concentration of DBP in the practical environment. The effect of DBP on survival, reproduction and growth was monitored. The parameters used to evaluate the environment effect on reproduction included the body length, moulting times, pregnancy times. The results showed that the low concentration of DBP had no significant (P 99.5 % purity) used in experiments was purchased from the Tianjin Bodi Chemical Reagent Company (Tianjin, China). Acetone (analytically pure) used in experiments was purchased from the Tianjin Jinbei Chemical Reagent Company (Tianjin, China). Stock solutions of DBP were prepared by ultrasonic dispersion in Acetone, recommended co solvent by OECD. Prior to each experiment, the stock solutions were prepared fresh then diluted to the desired concentration in medium. The final Acetone concentration in the aquaria was 10 μl/L. The experiment was performed with semi-static system. Culture medium was renewed and offsprings were discarded every day. Based on the residual concentrations of DBP reported by Hermann et al. [16] and Chen et al. [15], four selected
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concentration groups (0.2, 2, 20, 200 μg/L) and two control groups (water control, co solvent control) were used in the experiment. Experimental design included 3 parallel groups to each of the concentration groups and every parallel group had 10 parallel vessels. For the experiment, the neonates of D. magna, less than 24 h old, were used. One neonate was placed in an individual 50 ml container with 30 ml sample solution (M4 media). The experimental culture solutions were maintained at 22 ± 2 °C under a 16 h photoperiod with light intensity 1,100–2,500 lx. Experimental Daphnids were fed with Scenedesmus obliqnus with the density of 2.0 × 105– 3.0 × 105 cells • ml−1 every day. The experiment was continued for a period of 21 days and the culture medium was renewed per 24 h. Survival and growth was inspected and recorded everyday. And the body length of neonatal daphnia were inspected and recorded after 7, 14 and 21th day.
89.3 Results and Discussion The body length from head capsule to caudal spine of the neonatal daphnia (1st, the 7, 14 and 21th day) were measured and recorded, respectively. And the body lengths of D. magna treated by the selected concentrations of DBP at different ages are shown in Fig. 89.1. The statistical results showed that the body length of every group has no significant difference (P > 0.05). Therefore, there was no obvious effect on the growth of D. magna observed when the D. magna was exposed to the low dose of DBP during 21 days. The moulting time of D. magna exposed to DBP is shown in Fig. 89.2. As can be seen in Fig. 89.2, the significance analysis demonstrated that the moulting times
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