Sustained inhibition of rat myometrial gap junctions and contractions by lindane
- PDF / 2,234,554 Bytes
- 13 Pages / 610 x 792 pts Page_size
- 58 Downloads / 140 Views
BioMed Central
Open Access
Research
Sustained inhibition of rat myometrial gap junctions and contractions by lindane Rita K Loch-Caruso*1, Kay A Criswell1,2, Carmen M Grindatti1,3 and Kelly A Brant1 Address: 1Toxicology Program, Department of Environmental Health Sciences, University of Michigan, Ann Arbor, Michigan, USA, 2Current address: Pharmaceutical Research Division, Pfizer Global Research and Development, 2800 Plymouth Road, Ann Arbor, MI 48105, USA and 3Current address: NSF International, 789 N. Dixboro Road, Ann Arbor, MI 48105, USA Email: Rita K Loch-Caruso* - [email protected]; Kay A Criswell - [email protected]; Carmen M Grindatti - [email protected]; Kelly A Brant - [email protected] * Corresponding author
Published: 03 October 2003 Reproductive Biology and Endocrinology 2003, 1:62
Received: 03 June 2003 Accepted: 03 October 2003
This article is available from: http://www.RBEj.com/content/1/1/62 © 2003 Loch-Caruso et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
Abstract Background: Gap junctions increase in size and abundance coincident with parturition, forming an intercellular communication network that permits the uterus to develop the forceful, coordinated contractions necessary for delivery of the fetus. Lindane, a pesticide used in the human and veterinary treatment of scabies and lice as well as in agricultural applications, inhibits uterine contractions in vitro, inhibits myometrial gap junctions, and has been associated with prolonged gestation length in rats. The aim of the present study was to investigate whether brief exposures to lindane would elicit sustained inhibition of rat uterine contractile activity and myometrial gap junction intercellular communication. Methods: To examine effects on uterine contraction, longitudinal uterine strips isolated from late gestation (day 20) rats were exposed to lindane in muscle baths and monitored for changes in spontaneous phasic contractions during and after exposure to lindane. Lucifer yellow dye transfer between myometrial cells in culture was used to monitor gap junction intercellular communication. Results: During a 1-h exposure, 10 micro M and 100 micro M lindane decreased peak force and frequency of uterine contraction but 1 micro M lindane did not. After removal of the exposure buffer, contraction force remained significantly depressed in uterine strips exposed to 100 micro M lindane, returning to less than 50% basal levels 5 h after cessation of lindane exposure. In cultured myometrial myocytes, significant sustained inhibition of Lucifer yellow dye transfer was observed 24 h after lindane exposures as brief as 10 min and as low as 0.1 micro M lindane. Conclusion: Brief in vitro exposures to lindane have long-term effects on myometrial functions that are necessary for parturition, inhibiting spontaneous phasic contractions in late gestation rat ute
Data Loading...
