Synthesis and Application of Gold Nanoparticles Functionalized with Collagen Mimetic Peptides
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Synthesis and Application of Gold Nanoparticles Functionalized with Collagen Mimetic Peptides Xiao Mo, Seungju M. Yu Department of Materials Science & Engineering The Johns Hopkins University Baltimore, MD 21218 ABSTRACT Collagen is the principal tensile element of the extra-cellular matrix in animals and is the basic scaffold for cells and tissues. Abnormalities in its structure are known to result in a number of debilitating human diseases. Collagen mimetic peptides (CMPs) with repeat unit of (Pro-Hyp-Gly) are capable of forming right-handed triple-helical structures similar to that of the collagen triple helices. Recently, our group has shown that CMPs exhibit specific binding affinity to natural collagen under controlled thermal conditions. Using solid phase peptide synthesis, we have prepared a CMP cysteine derivative that was used to modify gold nanoparticles. Transmission electron microscopy (TEM) shows that the Cys-CMP functionalized gold nanoparticles have affinity to collagen fibers. We are investigating the interactions between Cys-CMP functionalized gold nanoparticles and collagen fibers. The Cys-CMP conjugated nanoparticles can potentially be used as a tool to visualize and understand unstable domains of collagen fibers which are related to a number of pathological conditions of extra cellular matrices. INTRODUCTION Collagen is the most abundant protein in animal and is the major structural protein in the extra-cellular matrix. Collagen molecules are composed of repeat units of (X-Y-Gly) sequence, where X and Y are often Proline (Pro) and 4-hydroxyproline (Hyp), respectively. In nature, three collagen chains intertwine around one another forming a tight triple helix. It has been reported that collagen mimetic peptides (CMP) with repeat units of (Pro-Hyp-Gly) have collagen-like triple helices [1]. Modification of collagen has received much attention since collagen has been widely used in drug delivery and tissue engineering [2]. Chemical coupling reaction [3] through amino acid side chain has been developed; however, chemical reactions between exogenous components and collagen molecules are hard to control since collagen molecules are very large and complex biopolymers. Our group has recently shown that CMP has strong propensity to associate with natural collagen in forming triple helices. We developed a “physical method” to attach exogenous component to collagen [4]. Fluorescence labeling was used for visualization of CMP-collagen complexes. But due to the intrinsic resolution of fluorescence microscope, the sub-micron structure of CMP-collagen complexes cannot be revealed. In this work, we present the synthesis of CMP gold nanoparticles conjugates and its interaction with collagen fibers. Gold nanoparticles serve as a visualization tool for CMPcollagen complexes in transmission electron microscopy (TEM).
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EXPERIMENTAL PROCEDURE Synthesis and characterization of gold nanoparticles: Gold nanoparticles were synthesized by citrate reduction method according to the literature [5]. Tri-sod
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