The Baculovirus Expression System A laboratory guide
The decision to write a book about the practical aspects of the baculovirus expression system stems from the numerous phone calls for help we have had, and from the many visitors to our labora tories requiring assistance to find the elusive polyhedrin-ne
- PDF / 23,001,386 Bytes
- 241 Pages / 439.32 x 666.12 pts Page_size
- 63 Downloads / 241 Views
The Baculovirus Expression System A laboratory guide L A . King School of Biological and Molecular Sciences, Oxford Polytechnic, Oxford, UK
R. D. Possee Natural Environment Research Council, Institute of Virology and Environmental Microbiology, Oxford, UK
m
SPRINGER-SCIENCE+BUSINESS MEDIA, B.V.
First e d i t i o n 1992 ©
1992 L . A . K i n g a n d R. D . Possee
Originally published by Chapman & H a l l i n 1992 Typeset i n IO/IIV2 Palatino b y Falcon Typographic A r t L t d , Edinburgh ISBN 978-94-010-5047-0 I S B N 978-94-011-2374-7 (eBook) D O I 10.1007/978-94-011-2374-7 A p a r t f r o m any fair dealing for the purposes of research or private study, or criticism or review, as permitted under the U K C o p y r i g h t Designs a n d Patents A c t , 1988, this p u b l i c a t i o n m a y not be reproduced, stored, or transmitted, i n any form or b y any means, w i t h o u t the p r i o r p e r m i s s i o n i n w r i t i n g of the publishers, or i n the case of reprographic reproduction only i n accordance w i t h the terms of the licences b y the C o p y r i g h t L i c e n s i n g A g e n c y i n the U K , or i n accordance w i t h the terms of licences issued b y the appropriate R e p r o d u c t i o n Rights O r g a n i z a t i o n outside the U K . E n q u i r i e s concerning reproduction outside the terms stated here should be sent to the publishers at the L o n d o n address p r i n t e d o n this page. The p u b l i s h e r makes no representation, express or i m p l i e d , w i t h regard to the accuracy of the i n f o r m a t i o n contained i n this book a n d cannot accept any legal responsibility or l i a b i l i t y for any errors or omissions that m a y be made. A catalogue record for this book is available from the B r i t i s h L i b r a r y L i b r a r y of Congress C a t a l o g i n g - i n - P u b l i c a t i o n data available
For Simon David
Contents
1 The baculoviruses 1.1 Introduction 1.2 Isolation and host range 1.3 Structure and classification 1.4 Baculovirus replication in vivo 1.5 Baculovirus replication in vitro 1.5.1 Baculovirus gene expression and replication 1.5.2 Baculovirus gene promoters 1.6 Genetic engineering of baculovirus insecticides 2 The development of baculovirus expression vectors 2.1 Introduction and historical perspective 2.2 The merits of the baculovirus expression system 2.2.1 Advantages 2.2.2 Disadvantages 2.3 General principles for inserting foreign genes into the baculovirus genome 2.4 Baculovirus transfer vectors 2.4.1 Polyhedrin promoter-based expression vectors 2.4.2 p10 promoter-based transfer vectors 2.4.3 Multiple expression vectors 2.4.4 Transfer vectors utilizing other baculovirus gene promoters 2.5 Selection of recombinant viruses 2.5.1 Selection of a polyhedrin-negative phenotype 2.5.2 Selection of f3-galactosidase-negative viruses
1 1 1 2 4 7
8 12 15
16 16 17 17 18 18 19 21 25 27 29 30 31 33
viii / Contents 2.5.3 Recombinant virus selection using dot-blot hybridization 2.5.4 Screening for a positive phenotype 2.5.5 Enhancing the numb
