Baculovirus and Insect Cell Expression Protocols
The third edition of this volume expands upon the previous two editions with new and up-to-date methods and protocols. Chapters include step-by-step procedures involved in quantifying cell growth, baculovirus infection and cell metabolism,
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David W. Murhammer Editor
Baculovirus and Insect Cell Expression Protocols Third Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Baculovirus and Insect Cell Expression Protocols Third Edition
Edited by
David W. Murhammer Department of Chemical & Biochemical Engineering, University of Iowa, Iowa City, IA, USA
Editor David W. Murhammer Department of Chemical & Biochemical Engineering University of Iowa Iowa City, IA, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3042-5 ISBN 978-1-4939-3043-2 (eBook) DOI 10.1007/978-1-4939-3043-2 Library of Congress Control Number: 2015952020 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media, LLC 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface Baculoviruses, which are a group of viruses that infect invertebrates, were first “discovered” in diseased silk worms in the 1500s, although the viral nature of this disease was not demonstrated until 1947. Subsequently, hundreds of other baculoviruses have been discovered. For example, the Autographa californica multiple nucleopolyhedrovirus (AcMNPV) was first isolated from the alfalfa looper (i.e., A. californica) insect species. AcMNPV is the most widely used and best characterized baculovirus and is known to infect many insect species in addition to A. californica, including Spodoptera frugiperda (fall armyworm) and Trichoplusia ni (cabbage looper). Furthermore, AcMNPV is the baculovirus that is usually used to produce recombinant baculoviruses for subsequent recombinant protein synthesis. Shangyin Gao and Thomas Grace independently established the first con
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