The DELLA proteins interact with MYB21 and MYB24 to regulate filament elongation in Arabidopsis
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RESEARCH ARTICLE
Open Access
The DELLA proteins interact with MYB21 and MYB24 to regulate filament elongation in Arabidopsis Huang Huang1*, Yilong Gong2, Bei Liu2, Dewei Wu3, Min Zhang2, Daoxin Xie4 and Susheng Song2*
Abstract Background: Gibberellin (GA) and jasmonate (JA) are two essential phytohormones for filament elongation in Arabidopsis. GA and JA trigger degradation of DELLAs and JASMONATE ZIM-domain (JAZ) proteins through SCFSLY1 and SCFCOI1 separately to activate filament elongation. In JA pathway, JAZs interact with MYB21 and MYB24 to control filament elongation. However, little is known how DELLAs regulate filament elongation. Results: Here we showed that DELLAs interact with MYB21 and MYB24, and that R2R3 domains of MYB21 and MYB24 are responsible for interaction with DELLAs. Furthermore, we demonstrated that DELLA and JAZ proteins coordinately repress the transcriptional function of MYB21 and MYB24 to inhibit filament elongation. Conclusion: We discovered that DELLAs interact with MYB21 and MYB24, and that DELLAs and JAZs attenuate the transcriptional function of MYB21 and MYB24 to control filament elongation. This study reveals a novel cross-talk mechanism of GA and JA in the regulation of filament elongation in Arabidopsis. Keywords: Gibberellin, Jasmonate, MYB21, MYB24, Filament elongation
Background Stamen, comprising a filament and an anther, is one of the plant reproductive organs. Disruptions in stamen development, such as in filament elongation, anther dehiscence, or pollen maturation, can cause male sterility [1, 2]. Numerous studies revealed that these processes are controlled by phytohormones, including jasmonate (JA) and gibberellin (GA) [3, 4]. JAs, including jasmonic acid and its oxylipin derivatives, play key roles in the regulation of plant developmental processes, such as root growth [5], stamen development [6], trichome initiation [7, 8], flowering [9], leaf senescence [10, 11], and apical hook formation [12– 14], and as well as control diverse defense responses to abiotic and biotic stresses [15–21]. JAs are perceived by F-box protein CORONATINE INSENSITIVE 1 (COI1) * Correspondence: [email protected]; [email protected] 1 Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing University of Agriculture, Beijing 102206, China 2 Beijing Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, RNA Center, College of Life Sciences, Capital Normal University, Beijing 100048, China Full list of author information is available at the end of the article
[22, 23],and subsequently induce the degradation of JASMONATE ZIM-DOMAIN (JAZ) proteins [24–26]. Mutants in Arabidopsis JA biosynthesis and signaling transduction, such as defective in anther dehiscence 1 (dad1), 13-lipoxygenase 3/4 (lox3 lox4), 12-oxophytodienoate reductase 3 (opr3), coi1–1, or overexpressing the JAZ dominant forms JAZ1Δ3A or JAZ10.4 lead to deficiency in late stamen development, including filament elongation, anther dehiscence and pollen ma
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