The lack of the cell division protein FtsZ induced generation of giant cells under acidic stress in cyanobacterium Synec
- PDF / 5,727,265 Bytes
- 14 Pages / 595.276 x 790.866 pts Page_size
- 32 Downloads / 160 Views
ORIGINAL ARTICLE
The lack of the cell division protein FtsZ induced generation of giant cells under acidic stress in cyanobacterium Synechocystis sp. PCC6803 Hidetaka Kohga1 · Yoshikazu Saito1 · Mirai Kanamaru1 · Junji Uchiyama1,2 · Hisataka Ohta1,2 Received: 25 November 2019 / Accepted: 23 October 2020 © Springer Nature B.V. 2020
Abstract Bacteria exposed to environmental stresses often exhibit superior acclimation abilities to environmental change. Acid treatment causes an increase in the cell length of the cyanobacterium Synechocystis sp. PCC6803 under light conditions. We aimed to elucidate the relationship between acidic stress and cell enlargement. After being synchronized under dark conditions, the cells were cultivated at different pH (pH 8.0 or pH 6.0) levels under light conditions. Synechocystis 6803 cells exhibited only cell growth occurred (cell volume expansion) and slow proliferation under the acidic condition. In the recovery experiment of the enlarged cells, they proliferated normally at pH 8.0, and the cell lengths decreased to the normal cell size under light conditions. Inhibition of cell division might be caused by acidic stress. To understand the effect of acidic stress on cell division, we evaluated the expression of FtsZ via Western blotting. The FtsZ concentration in cells was lower at pH 6.0 than at pH 8.0 and was not sufficient for cell division in the photoautotrophic conditions. ClpXP is well known as a regulator of the Z-ring dynamics in E. coli. The transcriptional level of four clpXP genes was upregulated approximately threefold at pH 6.0 after 24 h compared with that in cells grown at pH 8.0. The lack of FtsZ may be caused by the upregulation of clpXP expression under acidic condition. Therefore, ClpXP may participate in the degradation of FtsZ and be involved in the regulation of cell division via FtsZ under acidic stress in Synechocystis 6803. Keywords Cyanobacteria · Cell division · Low-pH · Stress response · FtsZ · ClpXP
Introduction Bacterial cell division is a highly coordinated dynamic process involving numerous cellular proteins that function together to divide a single cell and generate two daughter cells (Egan and Vollmer 2013). Many studies have been reported on Escherichia coli, focusing on the FtsZ protein. In general, FtsZ is a highly conserved essential protein in bacteria that plays a key role in bacterial cell division and Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11120-020-00792-1) contains supplementary material, which is available to authorized users. * Hisataka Ohta [email protected] 1
Department of Mathematics and Science Education, Graduate School of Science, Tokyo University of Science, 1‑3 Kagurazaka, Shinjuku‑ku, Tokyo 162‑8601, Japan
Department of Biology, Faculty of Science, Tokyo University of Science, 1‑3 Kagurazaka, Shinjuku‑ku, Tokyo 162‑8601, Japan
2
forms a Z-ring as the scaffold for the assembly of the other cell division components (Dai and Lutkenhaus 1992; Margolin 2005; Harry
Data Loading...
