Undaria pinnatifida soluble fiber regulates Angptl3-LPL pathway to lessen hyperlipidemia in mice
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ORIGINAL PAPER
Undaria pinnatifida soluble fiber regulates Angptl3-LPL pathway to lessen hyperlipidemia in mice Hong-Bo Xiao & Xiang-Yang Lu & Heng-Bo Zhang & Zhi-Liang Sun & Jun Fang
Received: 18 October 2012 / Accepted: 12 March 2013 # University of Navarra 2013
Abstract Angiopoietin-like protein 3 (Angptl3)–lipoprotein lipase (LPL) pathway may be a useful pharmacologic target for hyperlipidemia. The present study was conducted to test the effect of soluble fiber extracted from Undaria pinnatifida (UP), on hyperlipidemia in apolipoprotein E-deficient (ApoE−/−) mice. Forty mice were divided into four groups (n=10): control group (C57BL/6J mice), ApoE−/− mice group, and two groups of ApoE−/− mice treated with UP fiber
H.-B. Xiao (*) : Z.-L. Sun (*) College of Veterinary Medicine, Hunan Agricultural University, Furong District, Changsha 410128, China e-mail: [email protected] e-mail: [email protected] X.-Y. Lu Hunan Agricultural Bioengineering Research Institute, Changsha 410128, China H.-B. Zhang Furong District Red Cross Hospital, Changsha 410126, China Z.-L. Sun Biological Veterinary Drugs Branch, National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, China J. Fang College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China
(5 or 10 % per day). UP soluble fiber treatment significantly decreased plasma and hepatic total cholesterol, triglycerides levels, plasma low-density lipoprotein cholesterol, and malondialdehyde concentrations and increased plasma high-density lipoprotein cholesterol level and downregulated protein expression of Angptl3 concomitantly with upregulated protein expression of LPL. In addition, T0901317 caused elevated expression of hepatic Angptl3 protein, and the effect of T0901317 was also abrogated by UP soluble fiber in C57BL/6J mice. The present results suggest that the UP soluble fiber regulates Angptl3-LPL pathway to lessen hyperlipidemia in mice. Keywords Undaria pinnatifida . Soluble fiber . Angiopoietin-like protein 3 . Hyperlipidemia . Mice Abbreviations UP Undaria pinnatifida Angptl3 Angiopoietin-like protein 3 LPL Lipoprotein lipase TC Cholesterol TG Triglycerides LDL Low-density lipoprotein HDL-C High-density lipoprotein cholesterol MDA Malondialdehyde AOAC Association of Official Analytical Chemists ApoE−/− Apolipoprotein E knockout LXRs Liver X receptors
H.-B. Xiao et al.
Introduction Hyperlipidemia is marked by elevated plasma cholesterol (TC) and/or triglycerides (TG) levels. Angiopoietin-like protein 3 (Angptl3) is secreted by the liver. As a hormone-like protein involved in angiogenesis, Angptl3 regulates lipid metabolism. Administration of recombinant Angptl3 to Angptl3deficient mice and wild type mice increases the plasma TC and TG levels [12]. Angptl3 decreases very lowdensity lipoprotein TG clearance by inhibiting lipoprotein lipase (LPL) activity [20]. Therefore, Angptl3LPL pathway may be a useful pharmacologic target fo
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